Clenbuterol prevents or inhibits loss of specific mRNAs in atrophying rat skeletal muscle.
Clenbuterol prevents or inhibits loss of specific mRNAs in atrophying rat skeletal muscle.
复制标题
克伦特罗可防止或抑制萎缩的大鼠骨骼肌中特定 mRNA 的丢失。
DOI：
10.1152/ajpcell.1988.254.5.c657
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
F. Booth
中科院分区：
文献类型：
--
作者：
P. Babij;F. Booth
已知去神经支配或后肢悬吊均可降低成年大鼠骨骼肌rRNA、α -肌动蛋白mRNA和细胞色素c mRNA的含量。在本研究中，在对成年雌性大鼠进行为期7天的比目鱼和腓肠肌去神经支配期间，给予盐酸克仑特罗（一种合成代谢剂），完全阻止了正常情况下发生在去神经支配肌肉中的rRNA、α -肌动蛋白mRNA和细胞色素c mRNA的丢失。虽然克仑特罗抑制了后肢悬吊7天后比目鱼肌和腓肠肌α -肌动蛋白mRNA的大部分丢失，但克仑特罗对后肢悬吊骨骼肌rRNA和细胞色素c mRNA丢失的影响较小。克仑特罗对因去神经支配或悬浮液引起的萎缩肌肉中的蛋白质含量没有影响。这些数据表明克仑特罗可以维持萎缩的成年大鼠骨骼肌中某些rna的表达。
It is known that denervation or hindlimb suspension both decrease the content of rRNA, alpha-actin mRNA, and cytochrome c mRNA in adult rat skeletal muscle. In the present study, the provision of clenbuterol (an anabolic agent) to adult female rats during a 7-day period of denervation of the soleus and gastrocnemius muscles prevented entirely the loss of rRNA, alpha-actin mRNA, and cytochrome c mRNA that normally occurs in denervated muscle. Although clenbuterol inhibited most of the loss of alpha-actin mRNA that occurred in the soleus and gastrocnemius muscles after 7 days of hindlimb suspension, clenbuterol administration had less effect on preventing the loss of rRNA and cytochrome c mRNA in hindlimb suspended skeletal muscle. Clenbuterol had no effect on protein content in atrophied muscle resulting from denervation or suspension. These data suggest that clenbuterol can maintain the expression of certain RNAs in atrophying adult rat skeletal muscle.