Chemokine requirements for B cell entry to lymph nodes and Peyer's patches.
Chemokine requirements for B cell entry to lymph nodes and Peyer's patches.
复制标题
B细胞进入淋巴结和Peyer斑块的趋化因子要求。
DOI：
10.1084/jem.20020201
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发表时间：
2002-07-01
影响因子：
15.3
通讯作者：
Cyster, Jason G
中科院分区：
文献类型：
--
作者：
Okada, Takaharu;Ngo, Vu N;Ekland, Eric H;Forster, Reinhold;Lipp, Martin;Littman, Dan R;Cyster, Jason G
B细胞进入淋巴结和派尔集合淋巴结依赖于趋化因子受体信号传导，但涉及的主要趋化因子尚未确定。在这里，我们发现CXCR 4 −/− B细胞的归巢在CCL 19（ELC）和CCL 21（SLC）缺陷型淋巴结T细胞缺乏小鼠中受到抑制，但在野生型小鼠中没有。我们还发现CXCR 4可以促进T细胞归巢。使用活体显微镜，我们发现，B细胞粘附到高内皮微静脉（HEV）被破坏时，CCR 7和CXCR 4的预脱敏。在派伊尔集合淋巴结中，除了CCR 7/CXCR 4之外，B细胞进入还依赖于CXCR 5。CXCL 12（SDF 1）广泛地显示在HEV上，而CXCL 13（BLC）选择性地发现于派尔斑毛囊HEV上。这些发现确立了B细胞进入淋巴结和派尔集合淋巴结的主要趋化因子和趋化因子受体要求。
B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4−/− B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.