Assignment of the ets-related transcription factor E1A-F gene (ETV4) to human chromosome region 17q21.
Assignment of the ets-related transcription factor E1A-F gene (ETV4) to human chromosome region 17q21.
复制标题
将 ets 相关转录因子 E1A-F 基因 (ETV4) 分配给人类染色体区域 17q21。
DOI：
10.1006/geno.1995.1158
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发表时间：
1995
期刊：
影响因子：
4.4
通讯作者：
K. Fujinaga
中科院分区：
文献类型：
--
作者：
M. Isobe;F. Yamagishi;K. Yoshida;F. Higashino;K. Fujinaga
编码腺病毒E1 A增强子结合蛋白E1 A-F的cDNA已通过筛选HeLa细胞{λ} gt 11表达文库的E1 A-F位点特异性DNA结合而分离（4）。E1 A-F与ETS结构域具有序列同源性，ETS结构域是序列特异性DNA结合所需的区域，并且是所有ets癌基因成员所共有的。E1 A-F基因（ETV 4）是小鼠PEA 3多瘤病毒增强子增强子激活子（3）基因的人类同源物，PEA 3和E1 A-F之间的氨基酸序列具有94%的同一性（4），而小鼠PEA 3多瘤病毒增强子增强子激活子（3）基因是最近鉴定的ets癌基因成员。有趣的是，ets癌基因家族的两个成员已被证明参与几种肿瘤的染色体易位（3，5，9）。因此，了解ets相关序列在人类基因组中的位置，可能会为该基因与特定恶性肿瘤、其他遗传病变或易感性的可能关联提供线索。通过体细胞杂交分析和荧光原位杂交（FISH）相结合，我们已经定位ETV 4基因座染色体17 q21。
The cDNA encoding adenovirus E1A enhancer-binding protein E1A-F has been isolated by screening a HeLa cell {lambda}gt11 expression library for E1A-F site-specific DNA binding (4). E1A-F has sequence homology with the ETS domain, a region required for sequence-specific DNA binding and common to all ets oncogene members. It is suggested that the E1A-F gene (ETV4) is a human homologue of the murine PEA3 polyomarvirus enhancer enhancer activator (3) gene, a recently characterized ets oncogene member, because of 94% identity in amino acid sequence between PEA3 and E1A-F (4). Interestingly, two members of the ets oncogene family have been shown to be involved in chromosome translocations in several neoplasma (3, 5, 9). Thus knowing the position of an ets-related sequence in the human genome may provide clues to a possible association of that gene with particular malignancies, other genetic lesions, or predisposition. By a combination of somatic cell hybrid analysis and fluorescence in situ hybridization (FISH), we have mapped the ETV4 locus to chromosome 17q21.