Diets rich in polyphenols and vitamin A inhibit the development of type I autoimmune diabetes in nonobese diabetic mice.
Diets rich in polyphenols and vitamin A inhibit the development of type I autoimmune diabetes in nonobese diabetic mice.
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DOI：
10.1093/jn/137.5.1216
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发表时间：
2007-05
期刊：
影响因子：
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通讯作者：
S. Zunino;D. Storms;C. Stephensen
中科院分区：
文献类型：
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作者：
S. Zunino;D. Storms;C. Stephensen
I型青少年糖尿病的特征在于活化的T淋巴细胞和单核细胞浸润到胰腺的胰岛中，导致炎症和产生胰岛素的β细胞的进行性破坏。我们假设，喂养非肥胖糖尿病（NOD）小鼠饮食富含多酚或维生素A，这两种已知的免疫功能调节剂，将减少与I型糖尿病相关的自身免疫炎症过程。给NOD小鼠喂食对照饮食（C）和含有1%冻干葡萄粉（GP）或250 IU维生素A/g（VA; 0.262微摩尔乙酸视黄酯/g）食物的饮食。当血糖达到13.9 mmol/L或更高时，认为小鼠患有糖尿病并处死。到大约7月龄时，71%的C小鼠进展为糖尿病。1%葡萄粉组和VA组糖尿病发病率分别为33%（P < 0.05）和25%（P < 0.05）。在LPS刺激后，接受GP和VA的小鼠的脾细胞的TNF-α产生低于C小鼠（P < 0.05）。胰腺组织的组织学分析显示，与C小鼠相比，接受GP和VA的小鼠胰岛炎的严重程度显著降低。这些数据表明，富含多酚或维生素A的饮食对胰岛β细胞的自身免疫性炎症攻击具有保护作用，并有可能减少自身免疫性糖尿病的发病和发病机制。
Type I juvenile diabetes mellitus is characterized by the infiltration of activated T lymphocytes and monocytes into the islets of Langerhans of the pancreas, resulting in inflammation and progressive destruction of the insulin-producing beta cells. We hypothesized that feeding nonobese diabetic (NOD) mice diets rich in polyphenols or vitamin A, both known modulators of immune function, would decrease the autoimmune inflammatory process associated with type I diabetes. NOD mice were fed a control diet (C) and diets containing either 1% freeze-dried grape powder (GP) or 250 IU vitamin A/g (VA; 0.262 micromol retinyl acetate/g) of food. Mice were considered diabetic and killed when blood glucose reached 13.9 mmol/L or greater. By approximately 7 mo of age, 71% of C mice progressed to diabetes. Incidence of diabetes was reduced to 33% (P < 0.05) and 25% (P < 0.05) in mice receiving 1% dietary grape powder and VA, respectively. Splenocytes from mice receiving both GP and VA had lower TNF-alpha production after LPS stimulation than C mice (P < 0.05). Histological analysis of pancreatic tissue showed a significant reduction in the severity of insulitis in the mice receiving GP and VA compared with C mice. These data suggest that diets rich in polyphenols or vitamin A have protective effects against autoimmune inflammatory attack of the islet beta cells and have the potential to reduce the onset and pathogenesis of autoimmune diabetes.