Interaction of bovine blood clotting factor Va and its subunits with phosolipid vesicles
Interaction of bovine blood clotting factor Va and its subunits with phosolipid vesicles
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牛凝血因子Va及其亚基与磷脂囊泡的相互作用
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
T. Lindhout
中科院分区：
文献类型：
--
作者：
P. van de Waart;H. Bruls;H. Hemker;T. Lindhout
凝血酶活化因子Va和因子Va亚基结合到大体积囊泡的研究通过基于通过离心从本体溶液中分离磷脂结合蛋白的技术进行。该技术允许直接测量游离蛋白浓度。它的结论是，磷脂结合位点上的因子Va是位于一个基本的因子Va亚基与Mr 80 000（因子Va-LC）。磷脂囊泡的组成，钙浓度，pH值，和离子强度上的平衡常数因子Va和因子Va-LC-磷脂相互作用的影响进行了研究。因子Va和因子Va-LC与磷脂的结合需要带负电荷的磷脂的存在。进一步证明发生以下情况：（a）钙离子与因子Va和因子Va-LC竞争磷脂结合位点。(b)蛋白-磷脂相互作用的解离常数随离子强度的增加而增加，而磷脂囊泡的最大蛋白结合容量不受离子强度的影响。(c)当囊泡由磷脂酸组成时，因子Va-磷脂相互作用的解离常数取决于pH。它的结论是，因子Va-磷脂相互作用主要是静电的性质，其中带正电荷的蛋白质上的基团直接与净带负电荷的磷脂的磷酸基团相互作用。结果表明，因子Va，因子Xa和凝血酶原一样，具有外源性膜蛋白的特性。
: Thrombin-activated factor Va and factor Va subunit binding to large-volume vesicles was investigated by a technique based on the separation by centrifugation of phospholipid-bound protein from the bulk solution. This technique allows the direct measurement of free-protein concentration. It is concluded that the phospholipid binding site on factor Va is located on a basic factor Va subunit with Mr 80 000 (factor Va-LC). The effects of phospholipid vesicle composition, calcium concentration, pH, and ionic strength on the equilibrium constants of factor Va- and factor Va-LC-phospholipid interaction were studied. Factor Va and factor Va-LC binding to phospholipid requires the presence of negatively charged phospholipids. It is further demonstrated that the following occur: (a) Calcium ions compete with factor Va and factor Va-LC for phospholipid-binding sites. (b) The dissociation constant of protein-phospholipid interaction increases with the ionic strength, whereas the maximum protein-binding capacity of the phospholipid vesicle was not affected by ionic strength. (c) The dissociation constant for factor Va-phospholipid interaction depends on pH when the vesicle consists of phosphatidic acid. It is concluded that factor Va-phospholipid interaction is primarily electrostatic in nature, where positively charged groups on the protein directly interact with the phosphate group of net negatively charged phospholipids. The results suggest that factor Va, like factor Xa and prothrombin, has the characteristics of an extrinsic membrane protein.
通过停流光散射进行凝血酶原-膜结合的动力学和机制分析。
影响因子：
2.9
作者：
Wei,GJ;Bloomfield,VA;Resnick,RM;Nelsestuen,GL
通讯作者：
Nelsestuen,GL
血小板蛋白酶激活凝血因子 V。
DOI：
10.1172/jci110697
发表时间：
1982
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Kane,WH;Mruk,JS;Majerus,PW
通讯作者：
Majerus,PW