The metabolism of cholesterol and certain hormonal steroids by Treponema denticola
The metabolism of cholesterol and certain hormonal steroids by Treponema denticola
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DOI：
10.1016/j.steroids.2005.11.006
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发表时间：
2006-05-01
期刊：
影响因子：
2.7
通讯作者：
Soory, M
中科院分区：
文献类型：
--
作者：
Clark, DT;Soory, M
目的是研究齿垢密螺旋体的参考培养物和新鲜分离株是否能够5 α-还原并进一步代谢睾酮、4-雄烯二酮、孕酮、皮质酮、皮质醇或胆固醇。两个参考和五个新鲜分离的培养物的齿垢毛癣菌进行了孵育，无论是放射性标记或未标记的类固醇底物，在第一种情况下，产品进行了鉴定，薄层色谱法和气相色谱-质谱法在后者。所有底物均为5 α还原的。参考培养物和齿垢毛癣菌的新鲜分离株均呈现3 β-和17 β-羟基类固醇脱氢酶活性。得出的结论是，齿垢锥虫是能够类固醇代谢和假说进行了讨论，关于这种代谢的体内功能，包括，齿垢锥虫利用主机提供的类固醇作为生长因子和齿垢锥虫类固醇代谢作为毒力因子。(c)2005年爱思唯尔公司All rights reserved.
The aim was to investigate whether reference cultures and fresh isolates of Treponema denticola are able to 5 alpha-reduce and further metabolise testosterone, 4-androstenedione, progesterone, corticosterone, cortisol or cholesterol. TWO reference and five freshly isolated cultures of T denticola were incubated with either radiolabeled or unlabeled steroid substrates; in the first case products were identified by thin layer chromatography and in the latter by gas chromatography-mass spectroscopy. All the substrates were 5 alpha-reduced. Both reference cultures and fresh isolates of T denticola presented 3 beta- and 17 beta-hydroxy steroid dehydrogenase activity. It was concluded that T denticola was capable of steroid metabolism and hypotheses are discussed regarding the in vivo function of this metabolism including, T denticola utilising host supplied steroids as growth factors and T denticola steroid metabolism acting as a virulence factor. (c) 2005 Elsevier Inc. All rights reserved.