Neutralizing antibody response variation against dengue 3 strains
Neutralizing antibody response variation against dengue 3 strains
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DOI：
10.1002/jmv.21234
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发表时间：
2008-10-01
影响因子：
12.7
通讯作者：
Guzman, Maria G.
中科院分区：
文献类型：
--
作者：
Alvarez, Mayling;Pavon-Oro, Alequis;Guzman, Maria G.
评估一组人血清对同型病毒的7个毒株的中和抗体活性。血清取自登革热病毒3型（DENV - 3）免疫个体。包括在2000年以及2001 - 2002年哈瓦那疫情期间不同时间点分离出的两种DENV - 3基因型毒株。对一组从登革热（DF）和登革出血热（DHF）患者急性发病后16 - 18个月采集的20份恢复期晚期血清进行了研究。这些个体是在2001 - 2002年哈瓦那DENV - 3疫情期间感染的。除了从登革热病例中采集的4份血清外，其余所有血清都有二次登革热病毒1型/登革热病毒3型感染。通过空斑减少中和试验测定针对7种DENV - 3毒株的血清中和抗体滴度。血清样本同时进行检测。研究的血清对基因型III的DENV - 3毒株显示出比基因型V更高水平的中和抗体。有趣的是，对2001 - 2002年疫情末期分离出的DENV - 3毒株检测到更高水平的中和抗体。在2001 - 2002年疫情中，观察到中和抗体几何平均滴度（GMT）随着疫情发展呈上升趋势。一般来说，从登革热病例中采集的血清抗体水平更高。证明了针对包括相同基因型毒株在内的两种同源基因型检测的DENV - 3免疫血清的中和能力存在差异。观察结果表明病毒在疫情过程中发生了变化。这一发现对登革热发病机制和疫苗研发的影响需要加以考虑。
To evaluate the neutralizing antibody activity of a human sera panel against seven strains of the homotypic virus. Sera were collected from DENV-3 immune individuals. Two DENV-3 genotypes and strains isolated at different time-points during the 2000 and 2001-2002 Havana epidemics were included. A panel of 20 late convalescent sera collected 16-18 months after acute illness from DF and DHF patients are studied. These individuals were infected during the 2001-2002 Havana DENV-3 epidemic. All but four sera collected from DF cases had a secondary DENV-1/DENV-3 infection. Sera neutralizing antibody titer against the seven DENV-3 strains were determined by plaque reduction neutralization technique. Sera samples were tested simultaneously. Studied sera showed higher levels of neutralizing antibodies to DENV-3 strains of genotype III compared to genotype V. Interesting, higher levels of neutralizing antibodies were detected to DENV-3 strain isolated at the end of the epidemic 2001-2002. An increased tendency of GMT of neutralizing antibodies according to epidemic evolution was observed for the 20012002 outbreak. In general, antibody levels in sera collected from DF cases were higher. Differences in the neutralization capacity Of immune DENV-3 sera tested against two homologous genotypes including strains of the same genotype are demonstrated. Observed results Suggest that virus changed in the course of the epidemic. The implications of this finding in terms of dengue pathogenesis and vaccine development need to be considered.