Determination of histamine concentrations in plasma by liquid chromatography/electrochemistry.
Determination of histamine concentrations in plasma by liquid chromatography/electrochemistry.
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通过液相色谱/电化学测定血浆中的组胺浓度。
DOI：
10.1093/clinchem/32.10.1823
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发表时间：
1986
影响因子：
9.3
通讯作者：
P. Goldiner
中科院分区：
文献类型：
--
作者：
L. Hársing;H. Nagashima;D. Duncalf;E. Vizi;P. Goldiner
用Amberlite CG 50弱阳离子交换树脂从去蛋白血浆中提取组胺（[3 H]组胺的分析回收率为60.5%）。蒸发沉淀，再溶解样品中的组胺与邻苯二甲醛和2-巯基乙醇在pH 11.5下缩合。该加合物在等度条件下通过液相色谱分离，并在玻碳电极上在+0.4 V下氧化用于电化学检测。3-以甲基组胺为内标。检测到低至0.45 pmol的标准组胺冷凝物。88份人血浆中组胺浓度呈正态分布，平均值为7.20（SD 2.61）nmol/L。真实的和提取的组胺产生相似的流体动力学伏安图，外源性和内源性组胺在不同的移动的相或不同的色谱柱下产生相同的色谱特性。当样品与二胺氧化酶（EC 1.4.3.6）孵育时，标准品和提取的组胺具有相似的降解速率。已知量组胺加至合并血浆的分析回收率为97.7%（SD 22.3%）。组胺测定的批间和批内CV分别为9.0%和8.6%。
Histamine was extracted from deproteinized plasma with Amberlite CG 50 weak cation-exchange resin (analytical recovery of [3H]histamine, 60.5%). The eluate was evaporated and histamine in the redissolved sample was condensed with o-phthalaldehyde and 2-mercaptoethanol at pH 11.5. This adduct was separated by liquid chromatography under isocratic conditions and oxidized on a glassy carbon electrode at +0.4 V for electrochemical detection. 3-Methylhistamine was used as internal standard. As little as 0.45 pmol of standard histamine condensate was detected. The histamine concentration in 88 human plasma samples appeared to be normally distributed; its mean value was 7.20 (SD 2.61) nmol/L. Authentic and extracted histamine produced similar hydrodynamic voltammograms, and exogenous and endogenous histamine gave identical chromatographic characteristics with different mobile phases or different chromatographic columns. Standard and extracted histamine had similar degradation rates when samples were incubated with diamine oxidase (EC 1.4.3.6). Analytical recovery of known amounts of histamine added to pooled plasma was 97.7% (SD 22.3%). The inter- and intra-assay CVs for histamine determinations were 9.0% and 8.6%, respectively.