Characterization of mRNA profiles of the exosome-like vesicles in porcine follicular fluid
Characterization of mRNA profiles of the exosome-like vesicles in porcine follicular fluid
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DOI：
10.1371/journal.pone.0217760
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发表时间：
2019-06-12
期刊：
影响因子：
3.7
通讯作者：
Sugiura, Koji
中科院分区：
文献类型：
--
作者：
Matsuno, Yuta;Kanke, Takuya;Sugiura, Koji
细胞外囊泡如外来体含有几种类型的转录物，包括mRNA和微小RNA（miRNA），并且已经成为细胞间通讯的重要介质。在几种哺乳动物的卵泡中发现了外泌体样囊泡。虽然miRNA的含量已被广泛表征，但缺乏对其mRNA谱的详细研究。在这里，我们描述了猪模型中卵泡中外来体样囊泡的mRNA谱。使用Illumina HiSeq平台分析从猪卵泡液分离的外泌体样囊泡的mRNA含量，并与来自壁颗粒细胞（MGCs）的那些进行比较。生物信息学研究表明，外泌体mRNA富含那些编码参与代谢、磷脂酰肌醇-4，5-二磷酸3-激酶（PI 3 K）-蛋白激酶B（AKT）和促分裂原活化蛋白激酶（MAPK）途径的蛋白质。虽然外泌体样囊泡的mRNA谱类似于MGCs的mRNA谱，但囊泡含有在MGCs中几乎检测不到的mRNA。因此，虽然大多数囊泡可能是从MGCs分泌的，但一些可能来源于其他细胞类型，包括卵泡膜细胞和卵母细胞，以及非卵巢器官/组织的细胞。因此，mRNA谱揭示了卵巢卵泡中外泌体样囊泡的几个新特征。
Extracellular vesicles such as exosomes contain several types of transcripts, including mRNAs and micro RNAs (miRNAs), and have emerged as important mediators of cell-to-cell communication. Exosome-like vesicles were identified in the ovarian follicles of several mammalian species. Although the miRNA contents have been extensively characterized, the detailed investigation of their mRNA profiles is lacking. Here, we characterize the mRNA profiles of exosome-like vesicles in ovarian follicles in a pig model. The mRNA contents of the exosome-like vesicles isolated from porcine follicular fluid were analyzed and compared with those from mural granulosa cells (MGCs) using the Illumina HiSeq platform. Bioinformatics studies suggested that the exosomal mRNAs are enriched in those encoding proteins involved in metabolic, phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) -protein kinase B (AKT), and mitogen-activated protein kinase (MAPK) pathways. While the mRNA profile of the exosome-like vesicles resembled that of MGCs, the vesicles contained mRNAs barely detectable in MGCs. Thus, while the majority of the vesicles are likely to be secreted from MGCs, some may originate from other cell types, including theca cells and oocytes, as well as the cells of non-ovarian organs/tissues. Therefore, the mRNA profiles unveiled several novel characteristics of the exosome-like vesicles in ovarian follicles.