A strain-specific antigen in Japanese Helicobacter pylori recognized in sera of Japanese children
A strain-specific antigen in Japanese Helicobacter pylori recognized in sera of Japanese children
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DOI：
10.1128/cdli.12.11.1280-1284.2005
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发表时间：
2005-11-01
期刊：
影响因子：
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通讯作者：
Nakazawa, T
中科院分区：
文献类型：
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作者：
Okuda, M;Sugiyama, T;Nakazawa, T
对基于日本株高相对分子质量细胞相关蛋白(JHM-CAP)的酶免疫试验(EIA)和基于美国株高相对分子质量细胞相关蛋白(HM-CAP)的酶免疫试验(HM-CAP)进行了评价。本文对131例日本无症状儿童(0~21岁，平均5.5岁)的血清标本进行了检测，其中43例为幽门螺杆菌粪便抗原试验阳性，88例为阴性。JHM-CAP-EIA的敏感性为93.0%，明显高于HM-CAP EIA的67.4%。10岁以下儿童HM-CAP假阴性结果较多。免疫印迹分析表明，JHM-CAP而不是HM-CAP制剂具有JHM-CAP阳性血清识别的100 kDa抗原。结论：JHM-CAP EIA对日本儿童幽门螺杆菌感染的血清学诊断具有很高的准确性，JHM-CAP EIA较HM-CAP EIA具有较高的敏感性，这是由于日本菌株中存在一个100 kDa的抗原，该抗原可能在感染早期被宿主免疫系统识别。
An enzyme immuno assay (EIA) test based on Japanese strain-derived high-molecular-weight cell-associated proteins (JHM-CAP) was evaluated by comparing with a previously developed EIA test based on a U.S. strain-derived high-molecular-weight cell-associated proteins (HM-CAP). Serum samples of 131 Japanese asymptomatic children (mean age, 5.5 years; range, 0 to 21 years) were tested that include 43 positive and 88 negative children as judged by Helicobacter pylori stool antigen test (HpSA test). Both tests showed comparable and reliable specificities, but the sensitivity of JHM-CAP EIA, at 93.0%, was much higher than that of HM-CAP EIA, at 67.4%. More false-negative results of HM-CAP were obtained in children under 10 years of age. Immunoblot analysis revealed that the JHM-CAP but not the HM-CAP preparation had a 100-kDa antigen recognized by JHM-CAP positive sera. It was concluded that JHM-CAP EIA is highly accurate for the serodiagnosis of H. pylori infection in Japanese young children and that the high sensitivity of JHM-CAP EIA in contrast to HM-CAP EIA is due to the presence of a 100-kDa antigen in Japanese strains that may be recognized by the host immune system at an early stage of infection.