Magnetometric evaluation of the effects of man-made mineral fibers on the function of macrophages using the macrophage cell line RAW 264.7.
Magnetometric evaluation of the effects of man-made mineral fibers on the function of macrophages using the macrophage cell line RAW 264.7.
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DOI：
10.2486/indhealth.45.426
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发表时间：
2007-06
影响因子：
2
通讯作者：
K. Shibata;Yuichiro Kudo;M. Tsunoda;Mayuko Hosokawa;Y. Sakai;M. Kotani;Y. Aizawa
中科院分区：
文献类型：
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作者：
K. Shibata;Yuichiro Kudo;M. Tsunoda;Mayuko Hosokawa;Y. Sakai;M. Kotani;Y. Aizawa
利用肺泡巨噬细胞(AM)进行细胞磁学测量，评价人造矿物纤维(MMMF)的毒性效应。另一方面，最近，小鼠巨噬细胞系RAW 264.7问世，并已被用作AM的体外模型。这项研究的目的是确定使用原始264.7细胞的细胞磁测量是否可以用来评估MMMF的毒性效应。RAW 264.7细胞分别加入0、20、40、60微克/毫升的辛酸钾或碳化硅晶须或0、15、20、25微克/毫升的温石棉作为阳性对照，用细胞磁强法和乳酸脱氢酶测定法评价纤维的毒性作用。在这一比较中，AM也暴露在温石棉纤维(CF)中。在RAW 264.7细胞中，PT20、40、60或SiC20、40、60、CF15、20和25微g/ml与各自的对照组相比有明显的延迟松弛。在LDH测定中，PT20、40、60、SiC20、40、60和CF15、20、25微g/ml可显著增加细胞培养上清液中LDH的含量。在染毒CF20的AM中，25微g/ml组较对照组明显延迟松弛，LDH显著升高。导致显着差异的MMMF水平在细胞磁学和LDH中相似。在RAW 264.7细胞和AM中，引起细胞磁学和乳酸脱氢酶显著差异的Cf水平是相同的。我们的结果表明，使用RAW 264.7细胞的细胞磁测量足以评估暴露于MMMFS的细胞毒性。
The toxic effects of man-made mineral fibers (MMMFs) have been evaluated by cell magnetometry using alveolar macrophages (AMs). Recently, on the other hand, the murine macrophage cell line, RAW 264.7, became available and has been used as an in vitro model of AMs. The objective of this study was to determine whether or not cell magnetometry using RAW 264.7 cells can be used to evaluate the toxic effects of MMMFs. RAW 264.7 cells were exposed to one of the MMMFs, potassium octatitanate (PT) or silicon carbide whisker (SiC) at 0, 20, 40 and 60 microg/ml, or chrysotile as a positive control at 0, 15, 20 and 25 microg/ml. The toxic effects of fibers were evaluated by cell magnetometry and LDH assay. For this comparison, AMs were also exposed to chrysotile fibers (CF). In the RAW 264.7 cells exposed to PT 20, 40, 60 or SiC 20, 40, 60, CF 15, 20 and 25 microg/ml, significant delayed relaxation were observed compared with the respective control. In the LDH assay, significant increases in LDH in the supernatant of the cells exposed to PT 20, 40, 60, SiC 20, 40, 60 and CF 15, 20, 25 microg/ml were observed. In AMs exposed to CF 20, 25 microg/ml significant delayed relaxation and significant increases in LDH compared with the control were observed. The levels of MMMFs that induced significant differences were similar for cell magnetometry and LDH. The levels of CF that induced significant differences in cell magnetometry and LDH were identical for RAW 264.7 cells and AMs. Our results suggest that cell magnetometry using RAW 264.7 cells is adequate to evaluate the cytotoxicity of exposure to MMMFs.