2 NUCLEAR MUTATIONS THAT BLOCK MITOCHONDRIAL PROTEIN IMPORT IN YEAST
2 NUCLEAR MUTATIONS THAT BLOCK MITOCHONDRIAL PROTEIN IMPORT IN YEAST
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DOI：
10.1073/pnas.81.15.4819
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
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作者：
YAFFE, MP;SCHATZ, G
分离出两种对线粒体蛋白输入温度敏感的酵母突变体。每个菌株都含有一个突变，导致生长停滞和 .β 前体的积累。 37℃孵育后线粒体F1-ATP酶亚基。 C. 这些病变（mas1 和 mas2）是非等位基因和隐性的。携带任一突变的细胞仅在 37°C 下经过 2-3 代后才停止生长。 C. 导入 F1 .beta。 37° 的亚基。与野生型细胞相比，mas1 中的 C 速度慢 250 倍以上，mas2 中的 C 速度慢 15 倍以上。 23 度。 C，突变型和野生型细胞中的输入发生率相似。这2个突变还降低了其他蛋白质的输入率；然而，不同前体的输入在2个菌株中受到不同程度的影响。 mas1 和 mas2 中的温度敏感输入步骤发生在前体到达线粒体基质之前。
Two yeast mutants that are temperature-sensitive for import of mitochondrial proteins were isolated. Each strain contains a single mutation that results in arrest of growth and accumulation of precursor to the .beta. subunit of the mitochondrial F1-ATPase after incubation at 37.degree. C. These lesions (mas1 and mas2) are nonallelic and recessive. Cells harboring either mutation stop growing only after 2-3 generations at 37.degree. C. Import of the F1 .beta. subunit at 37.degree. C is more than 250 times slower in mas1 and 15 times slower in mas2 than in wild-type cells. At 23.degree. C, import occurs with similar rates in mutant and wild-type cells. The 2 mutations also reduce the rate of import of other proteins; however, import of different pecursors is affected to different degrees in the 2 strains. The temperature-sensitive step in import in both mas1 and mas2 occurs before arrival of precursors in the mitochondrial matrix.