ANTIOXIDANT POTENTIAL AND DNA DAMAGE PROTECTING ACTIVITY OF AQUEOUS EXTRACT FROM ARMILLARIA MELLEA
ANTIOXIDANT POTENTIAL AND DNA DAMAGE PROTECTING ACTIVITY OF AQUEOUS EXTRACT FROM ARMILLARIA MELLEA
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DOI：
10.1111/j.1745-4514.2010.00520.x
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发表时间：
2012-04-01
影响因子：
4
通讯作者：
Wang, Jian Wen
中科院分区：
文献类型：
--
作者：
Gao, Li Wen;Wang, Jian Wen
本研究以蜜环菌菌丝体为原料，采用不同的体外抗氧化体系，研究了蜜环菌菌丝体热水提取物的抗氧化活性。该提取物对脂质体氧化有较强的抑制作用，在1.02.0mg/mL浓度下，抑制率达85%以上。该提取物清除超氧阴离子自由基的IC 50值为1.03 mg/mL，清除羟自由基的IC 50值为0.66 mg/mL。在48 mg/mL浓度下，该提取物对1，1-二苯基-2-苦基羟自由基（DPPH）的清除率达到77.2%以上。此外，菲咯啉-铜络合物介导的DNA损伤的荧光发射被抑制和延迟的提取物。浓度为1.255.0mg/mL的提取物对Fenton介导的羟基自由基诱导的质粒pBR 322超螺旋DNA链断裂也有保护作用。甘露醇（34.91%，w/w）和多糖（26.94%，w/w）是提取物中发现的主要天然存在的成分，具有清除羟自由基的活性。此外，该提取物多糖对DPPH自由基和超氧阴离子自由基均有较强的清除作用，对微粒体脂质过氧化和羟自由基诱导的超螺旋DNA损伤也有较强的抗氧化作用，提示该提取物多糖具有一定的抗氧化活性。结果表明，A.蜜环菌提取物可用于在制药和食品工业中防止氧化和DNA损伤。
In this study, the antioxidant activities of hot-water extract from cultured mycelia of Armillaria mellea were assessed in different in vitro systems. The extract showed strongly inhibitory effect on microsomal liposome oxidation with an inhibition rate over 85% at 1.02.0 mg/mL. The extract was effective in scavenging superoxide radical in a concentration dependent fashion with IC50 value of 1.03 mg/mL whereas that in scavenging abilities of hydroxyl radical was 0.66 mg/mL. 1,1-Diphenyl-2-picrylhydracyl (DPPH) radical scavenging activity of the extract reached more than 77.2% at the concentrations of 48 mg/mL. Moreover, phenanthroline-copper complex-mediated chemiluminescent emission of DNA damage was both inhibited and delayed by the extract. The extract at 1.255.0 mg/mL also protected supercoiled DNA strand in plasmid pBR322 against scission induced by Fenton-mediated hydroxyl radical. Both mannitol (34.91%, w/w) and polysaccharides (26.94%, w/w) were major naturally occurring components found in the extract to have scavenging activity on hydroxyl radical. In addition, the polysaccharide from the extract displayed both strong scavenging activity on free radical (DPPH and superoxide radicals) and antioxidant effects against microsomal lipid peroxidation, hydroxyl radical-induced supercoiled DNA damage, which suggested that the activity might be derived partly from the polysaccharide of the extract. The results obtained indicated that A. mellea extract may be useful for preventing oxidation and DNA damage in pharmaceutical and food industries.