Imaging of human leukemic T‐Cell Xenografts in nude mice by radiolabeled monoclonal antibodies and F(ab')2 fragments
Imaging of human leukemic T‐Cell Xenografts in nude mice by radiolabeled monoclonal antibodies and F(ab')2 fragments
复制标题
使用放射性标记单克隆抗体和 F(ab)2 片段对裸鼠体内的人白血病 T 细胞异种移植物进行成像
作者：
A. Vacca;J. Mach;F. Buchegger;S. Carrel
与 T 淋巴细胞标记物（称为分化簇 CD5 和 CD2）发生反应的单克隆抗体 (MoAb) 用碘 131 (131I) 标记，并静脉注射到携带来自人 T 细胞白血病系 Ichikawa 的固体皮下异种移植物的裸鼠体内。 MoAb 抗 CD5 和抗 CD2 均产生良好的平均肿瘤与全身比率，分别为 3.8 和 5.1。通过使用其 F(ab')2 片段，这些比率进一步增加至 MoAb 抗 CD5 的 10.0 和 MoAb 抗 CD2 的 15.5。注射两种 MoAb 的 F(ab')2 片段后，可以通过外部扫描对肿瘤进行清晰的成像。将来自 MoAb 抗 CD2 的 F(ab')2 片段和识别人 T 细胞系 Jurkat 表达的抗原受体的克隆型决定簇 (Ti) 的第三个 MoAb 片段注射到携带脾内 Jurkat 异种移植物的小鼠中。 MoAb 抗 CD2 和抗 Ti 的平均肿瘤与全身比率分别为 7.5 和 4.1，证明了这两个片段在肿瘤组织中的选择性定位。这些体内实验结果可能为放射性标记的 MoAb 和片段在 T 细胞淋巴瘤和不同其他形式的 T 细胞恶性肿瘤患者的诊断和治疗中的潜在用途提供有用的信息。
Monoclonal antibodies (MoAb) that react with the T‐lymphocyte markers called cluster of differentiation CD5 and CD2 were labeled with iodine 131 (131I) and were injected intravenously in nude mice bearing solid subcutaneous xenografts derived from the human T‐cell leukemia line Ichikawa. Both MoAb anti‐CD5 and anti‐CD2 yielded favorable mean tumor to whole‐body ratios of 3.8 and 5.1, respectively. These ratios were further increased up to 10.0 for MoAb anti‐CD5 and 15.5 for MoAb anti‐CD2 by using their F(ab')2 fragments. The tumors could be imaged clearly by external scanning after injection of F(ab')2 fragments from both MoAb. F(ab')2 fragments from MoAb anti‐CD2 and of a third MoAb recognizing the clonotypic determinant (Ti) of the antigen receptor expressed by the human T‐cell line Jurkat were injected in mice bearing intrasplenic Jurkat xenografts. A selective localization of both fragments in tumor tissue was demonstrated with mean tumor to whole‐body ratios of 7.5 and 4.1 for MoAb anti‐CD2 and anti‐Ti, respectively. These in vivo experimental results may provide useful information for the potential use of radiolabeled MoAb and fragments in the diagnosis and treatment of patients with T‐cell lymphoma and different other forms of T‐cell malignancies.
通过单克隆抗体对人类肿瘤异种移植物进行放射免疫检测。
影响因子：
11.2
作者：
Herlyn,D;Powe,J;Alavi,A;Mattis,JA;Herlyn,M;Ernst,C;Vaum,R;Koprowski,H
通讯作者：
Koprowski,H