Immunohistochemical analysis of oxidative DNA damage in arsenic-related human skin samples from arsenic-contaminated area of China
Immunohistochemical analysis of oxidative DNA damage in arsenic-related human skin samples from arsenic-contaminated area of China
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DOI：
10.1016/j.canlet.2004.04.005
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发表时间：
2004-10-08
期刊：
影响因子：
9.7
通讯作者：
Yamanaka, K
中科院分区：
文献类型：
--
作者：
An, Y;Gao, ZL;Yamanaka, K
用8-oxodG单克隆抗体对28例砷相关的人皮肤肿瘤和20例砷无关的人皮肤癌中8-oxodG的出现进行了化学检测，以确定氧化应激的诱导是否参与砷引起的皮肤肿瘤的发生。8-oxodG在砷相关皮肤癌中的阳性率（28/28，100%）显著高于砷无关皮肤癌（3/20，15%）（χ 2检验P < 0.01）。此外，在所有砷相关的皮肤样品中，不仅在肿瘤组织中，而且在角化病和正常组织中检测到8-oxodG。这些结果提示氧化应激的诱导可能在砷致癌过程中起重要作用。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
The appearance of 8-oxo-2'-deoxyguanosine (8-oxodG) was examined immunohistochemically using an 8-oxodG-monoclonal antibody in 28 cases of arsenic-related human skin tumors and in 20 cases of arsenic-unrelated human skin cancer to determine if the induction of oxidative stress participates in skin tumorigenesis caused by arsenics. The rate of 8-oxodG-positive was significantly higher in arsenic-related human skin cancer (28 of 28, 100%) than in arsenic-unrelated human skin cancer (3 of 20, 15%, P < 0.01 by chi(2) test). Moreover, in all the arsenic-related skin samples, 8-oxodG was detected not only in tumor tissues but also in keratosis and normal tissues. These results suggest that the induction of oxidative stress m ay play an important role in arsenic carcinogenesis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.