Diversity in receptor-like kinase genes is a major determinant of quantitative resistance to Fusarium oxysporum f.sp matthioli
Diversity in receptor-like kinase genes is a major determinant of quantitative resistance to Fusarium oxysporum f.sp matthioli
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DOI：
10.1111/nph.12368
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发表时间：
2013-10-01
期刊：
影响因子：
9.4
通讯作者：
Diener, Andrew C.
中科院分区：
文献类型：
--
作者：
Cole, Stephanie J.;Diener, Andrew C.
拟南芥对枯萎病的抗性是一个多基因性状。RFO 3是6个数量性状基因座之一，它们分别代表了哥伦比亚-0（Col-0）的完全抗性和Taynuilt-0（Ty-0）的敏感性。我们发现RFO 3的Col-0和Ty-0等位基因代表了野生拟南芥中的两个常见变异，对FOM的抗性和易感性是这两种变体的祖先特征，对RFO 3的抗性是基因组中其他基因无法比拟的-广泛调查加入的多样性。Col-0中的一个受体样激酶（receptor-like kinase，RLK）基因负责RFO 3的抗性，而敏感的Ty-0等位基因编码两个RLK同源物。尖孢菌感染，根表达的RFO 3限制了FOM对血管系统的感染。RFO 3对FOM具有特异性抗性，但对其它两种十字花科真菌无抗性。RFO 3的身份、表达和特异性表明RFO 3代表模式识别受体（PRR）基因的多样性。RFO 3和先前发表的RFO 1的特征表明，RLK PRRs的多样性是野生植物群体中数量抗性的主要决定因素。
Resistance to wilt fungus Fusarium oxysporum f.sp. matthioli (FOM) is a polygenic trait in Arabidopsis thaliana. RFO3 is one of six quantitative trait loci accounting for the complete resistance of accession Columbia-0 (Col-0) and susceptibility of accession Taynuilt-0 (Ty-0).We find that Col-0 and Ty-0 alleles of RFO3 are representative of two common variants in wild Arabidopsis accessions, that resistance and susceptibility to FOM are ancestral features of the two variants and that resistance from RFO3 is unrivalled by other genes in a genome-wide survey of diversity in accessions. A single receptor-like kinase (RLK) gene in Col-0 is responsible for the resistance of RFO3, although the susceptible Ty-0 allele codes for two RLK homologs.Expression of RFO3 is highest in vascular tissue, which F. oxysporum infects, and root-expressed RFO3 restricts FOM infection of the vascular system. RFO3 confers specific resistance to FOM and provides no resistance to two other crucifer-infecting F. oxysporum pathogens.RFO3's identity, expression and specificity suggest that RFO3 represents diversity in pattern-recognition receptor (PRR) genes. The characteristics of RFO3 and the previously published RFO1 suggest that diversity in RLK PRRs is a major determinant of quantitative resistance in wild plant populations.