Biodegradation of 1,4-dioxane by a Flavobacterium
Biodegradation of 1,4-dioxane by a Flavobacterium
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DOI：
10.1007/s10532-010-9438-9
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发表时间：
2011-06
期刊：
影响因子：
3.6
通讯作者：
Bozhi Sun;K. Ko;J. Ramsay
中科院分区：
文献类型：
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作者：
Bozhi Sun;K. Ko;J. Ramsay
从受污染的地下水羽流中获得的土壤中富集了二恶烷降解菌群。富集的财团并不使用二恶烷作为碳和能源的唯一来源，而是在四氢呋喃（THF）存在下共代谢二恶烷。浓度高达 1000 ppm 的 THF 和二恶烷在约 2 周内被富集的聚体降解，在 1000 ppm 时可观察到更长的滞后期。然后在含有基础盐和葡萄糖作为碳源的琼脂平板上获得来自富集聚生体的三个菌落。三个菌落中只有一个能够降解二恶烷。在液体培养基中进一步富集该菌落，形成纯培养物，在 THF 降解后，在葡萄糖和共代谢降解的二恶烷上生长。该分离物的二恶烷降解速率和程度随着 THF 浓度的增加而增加。随后通过 16S rDNA 测序将该分离株鉴定为黄杆菌。使用聚合酶链反应（PCR）和变性梯度凝胶电泳（DGGE）对微生物种群进行分析，确定黄杆菌是富集群落中的优势物种，并且与其他两个不降解二恶烷的菌落不同。这是二恶烷降解黄杆菌的首次报道，该黄杆菌在系统发育上与任何先前鉴定的二恶烷降解剂不同。
A dioxane-degrading consortium was enriched from soil obtained from a contaminated groundwater plume. The enriched consortium did not use dioxane as the sole source of carbon and energy but co-metabolized dioxane in the presence of tetrahydrofuran (THF). THF and dioxane concentrations up to 1000 ppm were degraded by the enriched consortium in about 2 weeks with a longer lag phase observable at 1000 ppm. Three colonies from the enriched consortium were then obtained on agar plates containing basal salts and glucose as the carbon source. Only one of the three colonies was capable of dioxane degradation. Further enrichment of this colony in liquid media led to a pure culture that grew on glucose and co-metabolically degraded dioxane after THF degradation. The rate and extent of dioxane degradation of this isolate increased with increasing THF concentration. This isolate was subsequently identified as aFlavobacteriumby 16S rDNA sequencing. Using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis of microbial populations,Flavobacteriumwas determined to be the dominant species in the enriched consortium and was distinct from the two other colonies that did not degrade dioxane. This is the first report of a dioxane-degradingFlavobacteriumwhich is phylogenetically distinct from any previously identified dioxane degrader.