Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery
Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery
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DOI：
10.1016/s0378-5173(00)00452-x
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发表时间：
2000-11-04
影响因子：
5.8
通讯作者：
Audus, KL
中科院分区：
文献类型：
--
作者：
Foster, KA;Avery, ML;Audus, KL
本研究的目的是检查人类支气管下腺细胞系 Calu-3，并评估其作为代谢和转运模型研究药物向呼吸道上皮输送的潜力。目前的研究是使用在 Transwell 或多孔簇板中生长的 Calu-3 细胞进行的。 Calu-3 单层的 TEER 值使用 World Precision Instrument 伏欧计和 STX-2 电极测定。结果证实，Calu-3 细胞在空气界面条件下生长时形成紧密的单层，并在培养物中产生可观的 TEER 值。 Calu-3 单层上亲脂性小分子的渗透性数据表明，该细胞系是检查低分子量物质和异生物质转运的合适模型。还发现 Calu-3 细胞以极化方式流出 FITC-转铁蛋白 (MW 80 000)。还检查了 Calu-3 细胞的代谢能力。通过荧光试卤灵测定，P4501A1 和 P4502B 同工酶被确定为有功能，但不可诱导。数据表明，Calu-3 细胞系可能有助于研究支气管上皮细胞对呼吸道上皮药物输送机制的贡献。 (C) 2000 年由 Elsevier Science B.V. 出版
The objective of this research was to examine the human sub-bronchial gland cell line, Calu-3, and assess its potential as a metabolic and transport model to study drug, delivery to the respiratory epithelium. The present studies were conducted using Calu-3 cells grown in Transwells(R) or in multiwell cluster plates. TEER values for Calu-3 monolayers were determined using the World Precision Instrument Voltohmmeter and STX-2 electrode. The results confirmed that Calu-3 cells form tight monolayers and give appreciable TEER values in culture when grown under air-interface conditions. Permeability data for small lipophilic molecules across Calu-3 monolayers suggested that the cell line is a suitable model to examine the transport of low molecular weight substances and xenobiotics. Calu-3 cells were also found to efflux FITC-transferrin (MW 80 000) in a polarized manner. The metabolic capacity of Calu-3 cells was also examined. The P4501A1 and P4502B isozymes were determined to be functional, but not inducible, with fluorescent resorufin assays. The data indicated that the Calu-3 cell line may be useful for studying the contributions of bronchial epithelial cells to mechanisms of drug delivery at the respiratory epithelium. (C) 2000 Published by Elsevier Science B.V.