Effects of Residual Oil Fly Ash (ROFA) in Mice with Chronic Allergic Pulmonary Inflammation
Effects of Residual Oil Fly Ash (ROFA) in Mice with Chronic Allergic Pulmonary Inflammation
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DOI：
10.1177/0192623308317427
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发表时间：
2008-07-01
影响因子：
1.5
通讯作者：
Martins, Milton A.
中科院分区：
文献类型：
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作者：
Arantes-Costa, Fernanda M.;Lopes, Fernanda D. T. Q. S.;Martins, Milton A.
过渡金属，与PM的病理作用有关。这项研究的目的是研究ROFA内鼻内给药对慢性肺部过敏性炎症小鼠模型中肺部炎症，肺反应性和过量粘液产生的影响。 BALB/C小鼠接受了卵蛋白（OVA）溶液的腹膜内注射（第1和14天）。在第22、24、26和28天进行了OVA挑战。在挑战之后，小鼠被鼻内灌输给了ROFA。 48小时后，进行了肺反应能力。处死小鼠，并去除肺进行形态计量分析。暴露于OVA的小鼠在支气管血管空间（P <.001），肺反应性增加（P <.001）和上皮重塑（P = .003）中呈现嗜酸性粒细胞。 ROFA滴注提高了肺反应性（P = .004），并降低了气道上皮细胞的面积（P = .006）。 ROFA的组合滴注和OVA暴露会导致肺反应性值进一步增加（P = .043），而气道上皮的纤毛细胞数量减少（P = .017）。 PM暴露会导致肺部作用在患有慢性过敏性肺部炎症的小鼠中更强烈。
transition metals, which are involved in the pathological effects of PM. The objective of this study was to investigate the effects of intranasal administration of ROFA on pulmonary inflammation, pulmonary responsiveness, and excess mucus production in a mouse model of chronic pulmonary allergic inflammation. BALB/c mice received intraperitoneal injections of ovalbumin (OVA) solution (days 1 and 14). OVA challenges were performed on days 22, 24, 26, and 28. After the challenge, mice were intranasally instilled with ROFA. After forty-eight hours, pulmonary responsiveness was performed. Mice were sacrificed, and lungs were removed for morphometric analysis. OVA-exposed mice presented eosinophilia in the bronchovascular space (p < .001), increased pulmonary responsiveness (p < .001), and epithelial remodeling (p = .003). ROFA instillation increased pulmonary responsiveness (p = .004) and decreased the area of ciliated cells in the airway epithelium (p = .006). The combined ROFA instillation and OVA exposure induced a further increase in values of pulmonary responsiveness (p = .043) and a decrease in the number of ciliated cells in the airway epithelium (p = .017). PM exposure results in pulmonary effects that are more intense in mice with chronic allergic pulmonary inflammation.