Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus
Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus
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DOI：
10.1016/s0168-9452(00)00392-7
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发表时间：
2001-01-05
期刊：
影响因子：
5.2
通讯作者：
Prasad, MNV
中科院分区：
文献类型：
--
作者：
Nagalakshmi, N;Prasad, MNV
本文报道了铜处理过的双jugatus细胞中谷胱甘肽代谢和谷胱甘肽含量的酶以及金属结合肽的合成。随着铜浓度的增加，细胞中谷胱甘肽含量逐渐减少。蛋白质硫醇含量增加，而非蛋白质硫醇含量降低。细胞内的过氧化氢浓度起初升高，后来又下降。铜胁迫提高了γ -谷氨酰半胱氨酸合成酶、谷胱甘肽转移酶和谷胱甘肽过氧化物酶的活性，降低了谷胱甘肽还原酶的活性。这些结果表明，由于其抗氧化作用或作为植物螯合素合成的前体，铜改变了谷胱甘肽合成和利用之间的平衡。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
Enzymes of glutathione metabolism and GSH content in copper-treated Scenedesmus bijugatus cells and the synthesis of metal-binding peptides are reported in this investigation. Progressive depletion of GSH content in the cells was observed with increasing concentrations of copper. There was an increase in the protein thiol content while the non-protein thiol content decreased. There was an initial elevation and later decrease in the hydrogen peroxide level in the cells. Copper stress increased the activities of gamma -glutamylcysteine synthetase, GSH S-transferase and GSH-peroxidase and decreased the activity of GSSG-reductase. These results suggest that copper alters the equilibrium between synthesis and utilization of GSH either due to its antioxidant role or by serving as a precursor in the synthesis of phytochelatins. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.