Purification and localization of intraflagellar transport particles and polypeptides.
Purification and localization of intraflagellar transport particles and polypeptides.
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DOI：
10.1007/978-1-60761-376-3_11
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发表时间：
2009
影响因子：
--
通讯作者：
R. Sloboda
中科院分区：
文献类型：
--
作者：
R. Sloboda
几乎所有纤毛和鞭毛的生长和维持都依赖于鞭毛内运输（IFT）过程的正常运作。这包括大多数人类细胞的初级纤毛，它们处于细胞周期的间期或地相。研究IFT的模型系统是双鞭毛藻的鞭毛。正是在这种生物体中，IFT被首次发现，来自突变aChlamydomonasmutant的遗传数据首次将IFT过程与人类多囊肾病联系起来。本章提供的信息涉及从鞭毛中纯化IFT颗粒的程序，并使用光学和电子显微镜方法在鞭毛中定位这些颗粒及其相关的运动蛋白。
The growth and maintenance of almost all cilia and flagella are dependent on the proper functioning of the process of intraflagellar transport (IFT). This includes the primary cilia of most cells in humans that are in interphase or the Gophase of the cell cycle. The model system for the study of IFT is the flagella of the bi-flagellate green algaChlamydomonas. It is in this organism that IFT was first discovered, and genetic data from aChlamydomonasmutant first linked the process of IFT to polycystic kidney disease in humans. The information given in this chapter addresses procedures to purify IFT particles from flagella and localize these particles, and their associated motor proteins, in flagella using light and electron microscopic approaches.