Activation of murine T cells by toxic shock syndrome toxin-1. The toxin-binding structures expressed on murine accessory cells are MHC class II molecules.
Activation of murine T cells by toxic shock syndrome toxin-1. The toxin-binding structures expressed on murine accessory cells are MHC class II molecules.
复制标题
中毒性休克综合征毒素-1 激活小鼠 T 细胞。
作者：
T. Uchiyama;T. Tadakuma;K. Imanishi;M. Araake;S. Saito;X. Yan;H. Fujikawa;H. Igarashi;N. Yamaura
使用125 I-TSST-1研究了小鼠淋巴组织上存在的中毒性休克综合征毒素-1（TSST-1）结合结构。在0 ℃下与TSST-1孵育3小时的T耗尽的C57 BL/6脾细胞对脾T细胞具有促有丝分裂作用，表明前者细胞结合TSST-1并将其呈递给T细胞。在转染I-Ab基因的C57 BL/6脾B细胞和L细胞中观察到TSST-1结合活性，但在脾T细胞和对照L细胞中未观察到TSST-1结合活性。Scatchard图分析显示这些B细胞和转染子以相似的结合亲和力结合TSST-1。SDS-PAGE分析表明，C57 BL/6脾细胞和I-Ab阳性转染细胞的裂解液均含有一条与TSST-1结合的条带，并与I-Ab异源二聚体共迁移。在C57 BL/6中清楚地观察到TSST-1结合活性。BALB/c和C_3H/HeN脾细胞和转染I-Ab或I-Ak基因的L细胞不被多聚甲醛固定减少。125 I-TSST-1与三种脾细胞的结合被抗I-A抗体显著降低，但不被抗I-E抗体降低。C57 BL/6、C3 H/HeN和（C3 H/HeN x C57 BL/6）F1 T细胞分别在FT 6.2细胞、LT 1 -30-3细胞和它们中的任一种存在下被TSST-1激活以增殖和产生IL-2，但在对照L细胞存在下不活化。这些结果表明，I-A分子通过辅助细胞直接结合细胞表面上的TSST-1并将TSST-1的触发信号呈递给T细胞而起结构的作用。
Toxic shock syndrome toxin-1 (TSST-1)-binding structures present on murine lymphoid tissues were investigated by using 125I-TSST-1. T-depleted C57BL/6 spleen cells incubated with TSST-1 for 3 h at 0 degree C were mitogenic to splenic T cells, indicating that the former cells bind and present TSST-1 to T cells. TSST-1-binding activity was observed in C57BL/6 splenic B cells and L cells transfected with I-Ab genes, but not in splenic T cells and control L cells. Scatchard plot analysis showed that these B cells and transfectants bound TSST-1 with similar binding affinity. SDS-PAGE analysis showed that lysates of C57BL/6 spleen cells and the I-Ab-positive transfectants contain a single band which bound TSST-1 and comigrated with I-Ab heterodimers. TSST-1-binding activity observed clearly in C57BL/6. BALB/c, and C3H/HeN spleen cells and L cells transfected with I-Ab or I-Ak genes was not reduced by paraformaldehyde fixation. Binding of 125I-TSST-1 to the three spleen cells was markedly reduced by anti-I-A antibodies, but not by anti-I-E antibodies. C57BL/6, C3H/HeN, and (C3H/HeN x C57BL/6) F1 T cells were activated by TSST-1 to proliferate and produce IL-2 in the presence of FT6.2 cells, LT1-30-3 cells and either of them, respectively, but not in the presence of control L cells. These results indicate that I-A molecules function as the structures via that accessory cells directly bind TSST-1 on the cell surface and present a triggering signal of TSST-1 to T cells.
中毒性休克综合征毒素1的核苷酸和部分氨基酸序列。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Blomster-Hautamaa,DA;Kreiswirth,BN;Kornblum,JS;Novick,RP;Schlievert,PM
通讯作者：
Schlievert,PM
关节炎支原体衍生丝裂原刺激T细胞机制的研究。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bekoff,MC;Cole,BC;Grey,HM
通讯作者：
Grey,HM