Characterization of a vacuolar zinc transporter OZT1 in rice (Oryza sativa L.)
Characterization of a vacuolar zinc transporter OZT1 in rice (Oryza sativa L.)
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DOI：
10.1007/s11033-012-2162-2
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发表时间：
2013-02-01
影响因子：
2.8
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
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作者：
Lan, Hong-Xia;Wang, Zhou-Fei;Zhang, Hong-Sheng
CDF家族是在原核生物、真核生物和古细菌中已经鉴定的普遍存在的家族。该家族的成员是重要的重金属转运蛋白，将金属离子转运出细胞质。本研究从水稻中克隆了与大鼠锌转运蛋白2（ZnT-2）基因同源的水稻锌转运蛋白1（Oryza sativa Zn Transporter 1，OZT 1）全长cDNA。编码CDF家族蛋白的OZT 1与其他锌转运蛋白如RnZnT-2、HsZnT-8、RnZnT-8和AtMTP 1具有28.2%相似性和84.3%相似性，并且具有49.3%相似性和90.9%相似性。OZT 1在水稻不同组织中组成型表达。Zn 2+和Cd 2+处理显著诱导了粳稻日本晴和籼稻IR 26幼苗中OZT 1的表达。此外，当暴露于其他过量金属，如Cu 2+，Fe 2+和Mg 2+时，OZT 1表达也增加。亚细胞定位分析表明，OZT 1定位于液泡。OZT 1的异源表达提高了酵母对Zn ~（2+）和Cd ~（2+）胁迫的耐受性，但对Mg ~（2+）胁迫没有影响。OZT 1是一个CDF家族的液泡锌转运蛋白，具有对Zn ~（2+）和Cd ~（2+）胁迫的耐受性，对植物体内Zn、Cd等重金属的转运和体内平衡具有重要意义。
The CDF family is a ubiquitous family that has been identified in prokaryotes, eukaryotes, and archaea. Members of this family are important heavy metal transporters that transport metal ions out of the cytoplasm. In this research, a full length cDNA named Oryza sativa Zn Transporter 1 (OZT1) that closely related to rat ZnT-2 (Zn Transporter 2) gene was isolated from rice. The OZT1 encoding a CDF family protein shares 28.2 % similar to 84.3 % of identities and 49.3 % similar to 90.9 % of similarities with other zinc transporters such as RnZnT-2, HsZnT-8, RnZnT-8 and AtMTP1. OZT1 was constitutively expressed in various rice tissues. The OZT1 expression was significantly induced both in the seedlings of japonica rice Nipponbare and indica rice IR26 in response to Zn2+ and Cd2+ treatments. Besides, OZT1 expression was also increased when exposed to other excess metals, such as Cu2+, Fe2+ and Mg2+. Subcellular localization analysis indicated that OZT1 localized to vacuole. Heterologous expression of OZT1 in yeast increased tolerance to Zn2+ and Cd2+ stress but not the Mg2+ stress. Together, OZT1 is a CDF family vacuolar zinc transporter conferring tolerance to Zn2+ and Cd2+ stress, which is important to transporting and homeostasis of Zn, Cd or other heavy metals in plants.