Effects of ripasudil, a rho kinase inhibitor, on blood flow in the optic nerve head of normal rats.
Effects of ripasudil, a rho kinase inhibitor, on blood flow in the optic nerve head of normal rats.
复制标题
rho 激酶抑制剂瑞帕舒地尔对正常大鼠视神经乳头血流的影响。
DOI：
10.1007/s00417-018-4191-6
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Sugiyama K.
中科院分区：
文献类型：
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作者：
Wada Y;Higashide T;Nagata A;Sugiyama K.
PurposeTo评估局部给药的Ripasudil，rho激酶抑制剂，对血流在正常rates.MethodsRipasudil（0.4%）或安慰剂的视神经乳头（ONH）的正常棕色挪威大鼠的右眼，在一个双盲的方式。在单次滴注后20或40 min以及每天两次滴注7或14天之前和之后测量右眼ONH中的激光散斑血流图。在总面积（MA）、血管区域（MV）和组织区域（MT）中评价平均模糊率。眼内压（IOP），血压，眼灌注压（IOP），和心率也被记录在每个时间点。ResultsAfter一个单一的滴注，MV是显着大于40分钟比20分钟在利帕舒地尔组（P= 0.044），并显着低于安慰剂组（P= 0.023）。利帕舒地尔组滴注后40 min的MA和MV显著大于安慰剂组（分别为P= 0.022和P = 0.006）。持续滴注后，利帕舒地尔组的MA和MV在治疗7天和14天后较基线显著增加（均P < 0.05），MA、MV和MT显著高于安慰剂组（MA：7天和14天，P< 0.01; MV：7天，P= 0.003; 14天，P= 0.012; MT：7天，P= 0.046）。有没有显着的变化，眼压，血压，或眼压后，单次或连续instillations.ConclusionsTopical滴注利帕舒地尔增加周围的ONH在正常大鼠的眼睛的血流量。
PurposeTo evaluate the effect of topically administrated ripasudil, a rho kinase inhibitor, on blood flow in the optic nerve head (ONH) of normal rats.MethodsRipasudil (0.4%) or placebo was administered in the right eye of normal Brown Norway rats in a double-blind manner. Laser speckle flowgraphy was measured in the ONH of the right eye 20 or 40 min after a single instillation and before and after 7 or 14 days of twice daily instillation. Mean blur rate was evaluated in the total area (MA), the vessel region (MV), and the tissue region (MT). Intraocular pressure (IOP), blood pressure, ocular perfusion pressure (OPP), and heart rate were also recorded at each time point.ResultsAfter a single instillation, MV was significantly larger at 40 min than 20 min in the ripasudil group (P= 0.044) and was significantly lower in the placebo group (P= 0.023). MA and MV 40 min after instillation were significantly larger in the ripasudil group than in the placebo group (P= 0.022 andP= 0.006, respectively). After continuous instillation, MA and MV in the ripasudil group significantly increased from baseline after 7 and 14 days of treatment (bothP< 0.05) and MA, MV, and MT were significantly higher than in the placebo group (MA: 7 and 14 days,P< 0.01; MV: 7 days,P= 0.003, and 14 days,P= 0.012; MT: 7 days,P= 0.046). There were no significant changes in IOP, blood pressure, or OPP after single or continuous instillation.ConclusionsTopical instillation of ripasudil increased blood flow around the ONH in the eyes of normal rats.