TAP, a novel T cell-activating protein involved in the stimulation of MHC-restricted T lymphocytes
TAP, a novel T cell-activating protein involved in the stimulation of MHC-restricted T lymphocytes
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TAP，一种新型 T 细胞激活蛋白，参与刺激 MHC 限制性 T 淋巴细胞
DOI：
--
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发表时间：
1986
影响因子：
15.3
通讯作者：
B. Benacerraf
中科院分区：
文献类型：
--
作者：
K. Rock;E. Yeh;C. Gramm;S. Haber;H. Reiser;B. Benacerraf
已经产生了五种单克隆抗体，并用于表征TAP（T细胞活化蛋白），一种新的功能性小鼠T细胞膜抗原。TAP分子是由T细胞合成的12 kD蛋白。通过抗体交叉阻断，它似乎与T细胞膜上的16 kD蛋白密切相关，该蛋白也用新型mAb鉴定。这些分子明显不同于先前描述的主要良好表征的鼠T细胞抗原。与TAP结合的抗体可导致MHC限制性抗原特异性诱导物T细胞杂交瘤的活化，其在量级上等同于最大抗原或凝集素刺激。这是可溶性抗体的直接作用，不需要辅助细胞或其他因子。活化性抗TAP mAb在辅助细胞或IL-1存在下对正常异质性T淋巴细胞也具有促有丝分裂作用。此外，观察到这些抗体调节特异性免疫刺激。因此，活化性抗TAP mAb与T细胞的抗原特异性刺激协同作用，而非活化性抗TAP mAb抑制抗原驱动的活化。这些观察结果表明，TAP分子可能参与生理性T细胞活化。TAP已知的生理触发结构，T3- T细胞受体复合物，被认为是可能的关系。TAP在70%的外周T细胞上表达，因此定义了一个主要的T细胞亚群，使其可能成为鼠亚群特异性活化蛋白的第一个例子。
Five mAbs have been generated and used to characterize TAP (T cell activating protein) a novel, functional murine T cell membrane antigen. The TAP molecule is a 12-kD protein that is synthesized by T cells. By antibody crossblocking, it appears to be closely associated with a 16- kD protein on the T cell membrane also identified with a novel mAb. These molecules are clearly distinct from the major well-characterized murine T cell antigens previously described. Antibody binding to TAP can result in the activation of MHC-restricted, antigen-specific inducer T cell hybridomas that is equivalent in magnitude to maximal antigen or lectin stimulation. This is a direct effect of soluble antibody and does not require accessory cells or other factors. The activating anti-TAP mAbs are also mitogenic for normal heterogeneous T lymphocytes in the presence of accessory cells or IL-1. In addition, these antibodies are observed to modulate specific immune stimulation. Thus, the activating anti-TAP mAbs synergise with antigen-specific stimulation of T cells, while a nonactivating anti-TAP mAb inhibits antigen driven activation. These observations suggest that the TAP molecule may participate in physiologic T cell activation. The possible relationship of TAP to known physiologic triggering structures, the T3- T cell receptor complex, is considered. TAP is expressed on 70% of peripheral T cells and therefore defines a major T cell subset, making it perhaps the first example of a murine subset-specific activating protein.
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影响因子：
4.4
作者：
A. Bhattacharya;M. Dorf;T. Springer
通讯作者：
A. Bhattacharya;M. Dorf;T. Springer
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Rock,KL
通讯作者：
Rock,KL
DOI：
10.1016/s0021-9258(18)32549-3
发表时间：
1983-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jannie Borst;Stephen Alexander;John Elder;Cox TerhorstSn
通讯作者：
Jannie Borst;Stephen Alexander;John Elder;Cox TerhorstSn
DOI：
10.1073/pnas.79.14.4395
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEUER, SC;SCHLOSSMAN, SF;REINHERZ, EL
通讯作者：
REINHERZ, EL
一种人类 T 淋巴细胞分化标记物，由阻止 E-玫瑰花结形成的单克隆抗体定义。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Howard,FD;Ledbetter,JA;Wong,J;Bieber,CP;Stinson,EB;Herzenberg,LA
通讯作者：
Herzenberg,LA