Design of Gold Nanoparticle-Based Colorimetric Biosensing Assays
Design of Gold Nanoparticle-Based Colorimetric Biosensing Assays
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DOI：
10.1002/chin.200852275
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Weian Zhao;M. Brook;Yingfu Li
中科院分区：
文献类型：
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作者：
Weian Zhao;M. Brook;Yingfu Li
基于金纳米粒子（AuNP）的比色生物传感分析由于其简单性和多功能性最近在诊断应用中引起了相当大的关注。这篇小综述总结了这一领域的最新进展，并试图就如何设计此类检测提供一般指导。基于AuNP的比色传感平台的关键是通过使用感兴趣的生物过程（或分析物）来控制胶体AuNP分散和聚集阶段。平衡颗粒间吸引力和排斥力的能力，决定了AuNP是否稳定或聚集，因此，溶液的颜色，是这种系统设计的核心。在这些测定中的AuNP聚集可以由“颗粒间交联”机制诱导，其中颗粒间键合形成的疏水益处克服颗粒间排斥力。或者，AuNP聚集可以通过在“非交联-聚集”机制中的胶体稳定性的受控损失来引导。在这种情况下，由于表面性质的变化，货车德瓦尔斯吸引力克服了颗粒间的排斥力。使用有代表性的例子，我们说明了一般的策略，通常用于控制金纳米粒子的聚集和分散在金纳米粒子为基础的比色测定。了解在这些系统中发挥重要作用的因素不仅可以为设计基于AuNP的比色分析提供指导，还可以促进在纳米组装，生物科学以及胶体和聚合物科学等领域利用这些原理的研究。
Gold nanoparticle (AuNP)-based colorimetric biosensing assays have recently attracted considerable attention in diagnostic applications due to their simplicity and versatility. This Minireview summarizes recent advances in this field and attempts to provide general guidance on how to design such assays. The key to the AuNP-based colorimetric sensing platform is the control of colloidal AuNP dispersion and aggregation stages by using biological processes (or analytes) of interest. The ability to balance interparticle attractive and repulsive forces, which determine whether AuNPs are stabilized or aggregated and, consequently, the color of the solution, is central in the design of such systems. AuNP aggregation in these assays can be induced by an “interparticle-crosslinking” mechanism in which the enthalpic benefits of interparticle bonding formation overcome interparticle repulsive forces. Alternatively, AuNP aggregation can be guided by the controlled loss of colloidal stability in a “noncrosslinking-aggregation” mechanism. In this case, as a consequence of changes in surface properties, the van der Waals attractive forces overcome interparticle repulsive forces. Using representative examples we illustrate the general strategies that are commonly used to control AuNP aggregation and dispersion in AuNP-based colorimetric assays. Understanding the factors that play important roles in such systems will not only provide guidance in designing AuNP-based colorimetric assays, but also facilitate research that exploits these principles in such areas as nanoassembly, biosciences and colloid and polymer sciences.