Glycosyl-phosphatidylinositol-phospholipase type D: A possible candidate for the generation of second messengers
Glycosyl-phosphatidylinositol-phospholipase type D: A possible candidate for the generation of second messengers
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DOI：
10.1006/bbrc.1997.6475
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发表时间：
1997-04-17
影响因子：
3.1
通讯作者：
VarelaNieto, I
中科院分区：
文献类型：
--
作者：
Jones, DR;Avila, MA;VarelaNieto, I
膜结合糖基磷脂酰肌醇已被证明是肌醇磷酸聚糖第二信使的前体。提取人肝膜和纯化的系列薄层层析显示三种糖脂，其与糖基磷脂酰肌醇从大鼠肝脏共迁移。这些磷脂组分对亚硝酸处理和牛血清中糖基磷脂酰肌醇特异性磷脂酶D的水解部分敏感。同时，发现从大鼠肝脏分离的糖基-磷脂酰肌醇是产生生物活性肌醇磷酸聚糖物质的酶的底物（通过测量鸡胚耳蜗前庭神经节内蛋白激酶A活性的抑制和细胞增殖的刺激来确定）。该分子被抗肌醇磷酸聚糖抗体识别。因此，我们建议，糖基磷脂酰肌醇特异性磷脂酶D可能涉及细胞信号。(C)北京：科学出版社.
Membrane associated glycosyl-phosphatidylinositols have been shown to be the precursors of inositol phosphoglycan second messengers. Extraction of human liver membranes and purification by serial thin layer chromatography revealed three glycolipids which co-migrate with glycosyl phosphatidylinositol from rat liver. These lipidic fractions were partially sensitive to treatment with nitrous acid and to hydrolysis by glycosyl-phosphatidylinositol-specific phospholipase D from bovine serum. In parallel, glycosyl-phosphatidylinositol isolated from rat liver was found to be a substrate for the enzyme generating a biologically active inositol phosphoglycan species (determined by measuring inhibition of protein kinase A activity and stimulation of cell proliferation within the chicken embryo cochleovestibular ganglion). This molecule was recognised by an anti-inositol phosphoglycan antibody. Hence, we propose that glycosyl-phosphatidylinositol-specific phospholipase D could be implicated in cellular signalling. (C) 1997 Academic Press.