Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea
Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea
复制标题
紫花针茅谷胱甘肽还原酶基因的分子克隆和表征
DOI：
10.1016/j.bbrc.2017.12.054
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发表时间：
2018
影响因子：
3.1
通讯作者：
Yang Yongping
中科院分区：
文献类型：
--
作者：
Wang Qiuli;Pu Yanan;Yang Danni;Yin Xin;He Zhaorong;Yang Yunqiang;Yang Yongping
活性氧是非生物胁迫中的一个重要因子，过量的活性氧对植物有害。谷胱甘肽还原酶（GR）在植物体内清除活性氧（ROS）中起重要作用。本研究从紫针茅中克隆了一个GR基因，命名为SpGR。该基因的开放阅读框全长1497 bp，编码498个氨基酸。亚细胞定位分析表明，SpGR定位于质膜和细胞核。SpGR的表达受冷、盐和干旱胁迫的诱导。功能分析表明，SpGR在拟南芥中的异位表达使拟南芥对盐胁迫的耐受性增强，但在低温和干旱胁迫下没有差异。盐胁迫下，转基因植株产生更多的GR，将GSSG还原为GSH，以清除活性氧。因此，SpGR可能是植物抗非生物胁迫的候选基因。
Reactive oxygen species (ROS) are a key factor in abiotic stresses; excess ROS is harmful to plants. Glutathione reductase (GR) plays an important role in scavenging ROS in plants. Here, aGRgene, namedSpGR, was cloned fromStipa purpureaand characterized. The full-length open reading frame was 1497 bp, encoding 498 amino acids. Subcellular localization analysis indicated that SpGR was localized to both the plasma membrane and nucleus. The expression ofSpGRwas induced by cold, salt, and drought stresses. Functional analysis indicated that ectopic expression of SpGR inArabidopsis thalianaresulted in greater tolerance to salt stress than that of wild-type plants, but no difference under cold or drought treatments. The results of GR activity and GSSG and GSH content analyses suggested that, under salt stress, transgenic plants produced more GR to reduce GSSG to GSH for scavenging ROS than wild-type plants. Therefore,SpGRmay be a candidate gene for plants to resist abiotic stress.