Framework nucleic acid-mediated pull-down microRNA detection with hybridization chain reaction amplification
Framework nucleic acid-mediated pull-down microRNA detection with hybridization chain reaction amplification
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框架核酸介导的下拉 MicroRNA 检测与杂交链式反应扩增
DOI：
10.1021/acsabm.8b00278
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发表时间：
2018
影响因子：
4.7
通讯作者：
Gang Liu
中科院分区：
文献类型：
--
作者：
Xiangmeng Qu;Mingshu Xiao;Fan Li;Wei Lai;Li Li;Yi Zhou;Chenglie Lin;Qian Li;Zhilei Ge;Yanli Wen;Hao Pei;Gang Liu
由于预后不良，胃癌仍然是世界范围内死亡率较高的疾病。此前的研究表明，微小RNA（miRNA）是胃癌早期诊断的有效生物标志物。为了实现相关 miRNA 的灵敏检测，以改善胃癌的早期诊断、分类和生存预后，我们开发了一种框架核酸 (FNA) 介导的微阵列，用于多种 miRNA 的定量分析。通过合理设计不同尺寸的FNA，我们系统地调节了DNA探针的表面密度和横向相互作用，这为生物传感接口的杂交效率和动力学的可编程定制提供了有效的手段。我们发现杂交效率随着 FNA 大小的增加而增加，并且最适合 FNA-17。结合杂交链式反应扩增策略，这种建立的 FNA 微阵列可以作为超灵敏和选择性分析平台，用于同时多重检测胃癌中的 miRNA（例如 FNA-miR-652、FNA-miR-627 和 FNA-miR-629）生物标志物。
Gastric cancer remains a disease of high mortality worldwide due to its poor prognosis. Previous studies have shown that microRNAs (miRNAs) are effective biomarkers for early diagnosis of gastric cancer. To realize sensitive detection of related miRNAs for improved early diagnosis, classification, and survival prognosis of gastric cancer, herein we developed a framework nucleic acid (FNA)-mediated microarray for quantitative analysis of multiple miRNAs. By rationally designing FNA with different sizes, we systematically modulated the surface density and lateral interactions of DNA probes, which provides an effective means for programmable tailoring of the hybridization efficiency and kinetics of the biosensing interface. We found that the hybridization efficiency was increased along with the size of the FNA and was optimum for FNA-17. In combination with the hybridization chain reaction amplification strategy, this established FNA microarray can serve as an ultrasensitive and selective analytical platform for simultaneous multiplexed detection of miRNA (e.g., FNA-miR-652, FNA-miR-627, and FNA-miR-629) biomarkers in gastric cancer.