An immunocytochemical marker for hamster retinal ganglion cells.
An immunocytochemical marker for hamster retinal ganglion cells.
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仓鼠视网膜神经节细胞的免疫细胞化学标记物。
DOI：
10.1007/bf01181558
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Frost,DO
中科院分区：
文献类型：
--
作者：
Bhide,PG;West,WC;Fry,KR;Frost,DO
我们研究了特异性和发展的时间进程的标记视网膜神经节细胞在叙利亚仓鼠的单克隆抗体AB 5。在成年仓鼠中，AB 5选择性地标记神经节细胞层中的胞体、内丛状层中的树突和神经纤维层中的轴突。当在AB 5免疫细胞化学之前用Dil逆行标记视网膜神经节细胞时，神经节细胞层中所有逆行标记的视网膜神经节细胞都是AB 5免疫反应性的，表明AB 5标记该层中所有类别的神经节细胞。在新生儿视神经横断导致视网膜神经节细胞耗竭的视网膜中，AB 5不标记任何胞体或突起，表明AB 5特异性标记视网膜神经节细胞。在发展过程中，AB 5标签首先出现在出生后第12天（P12; PO=出生后第一个24小时）的神经节细胞层中的细胞体的弱染色，并获得了出生后第14天在成人中看到的染色模式。从发病的AB 5免疫反应，AB 5标记的胞体的大小不等，存在于整个视网膜表面。虽然AB 5标记了视网膜神经纤维层中的视网膜神经节细胞轴突，但在所检查的任何年龄，它都不标记大脑中的视神经或视网膜神经节细胞轴突。AB 5标记也被发现是兼容的溴脱氧尿苷免疫细胞化学，因此，用于确定时间的仓鼠视网膜神经节细胞的生成。
We examined the specificity and developmental time course of the labelling of retinal ganglion cells in Syrian hamsters by a monoclonal antibody AB5. In adult hamsters, AB5 selectively labelled somata in the ganglion cell layer, dendrites in the inner plexiform layer and axons in the nerve fibre layer. When retinal ganglion cells were retrogradely labelled with Dil prior to AB5 immunocytochemistry, all of the retrogradely labelled retinal ganglion cells in the ganglion cell layer were AB5 immunoreactive, indicating that AB5 labels all classes of ganglion cell in that layer. In retinae depleted of retinal ganglion cells by neonatal optic nerve transections, AB5 did not label any somata or processes, indicating that AB5 specifically labels retinal ganglion cells. During development, AB5 labelling first appeared as a weak staining of cell bodies in the ganglion cell layer on postnatal day 12 (P12; PO=first 24 h following birth) and acquired the staining pattern seen in the adult by postnatal day 14. From the onset of AB5 immunoreactivity, AB5-labelled somata of varying sizes were present across the entire retinal surface. Although AB5 labelled retinal ganglion cell axons in the nerve fibre layer of the retina it did not label the optic nerve or retinal ganglion cell axons in the brain at any age examined. AB5 labelling was also found to be compatible with bromodeoxyuridine immunocytochemistry and, therefore, useful for determining the time of generation of hamster retinal ganglion cells.