Measuring Mitochondrial Calcium Fluxes in Cardiomyocytes upon Mechanical Stretch-Induced Hypertrophy.
Measuring Mitochondrial Calcium Fluxes in Cardiomyocytes upon Mechanical Stretch-Induced Hypertrophy.
复制标题
测量机械拉伸引起的心肌肥大时心肌细胞中的线粒体钙通量。
DOI：
10.1007/978-1-0716-2217-9_15
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Matter,MichelleL
中科院分区：
文献类型：
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作者：
Ramaccini,Daniela;Giorgi,Carlotta;Matter,MichelleL
钙Ca2+调节是许多细胞功能的关键组成部分。在心肌细胞中，Ca2+调节兴奋-收缩耦合并影响参与细胞代谢和细胞存活的信号级联。线粒体Ca2+的长期失调导致心肌细胞功能失调、细胞凋亡和最终心力衰竭。VEGF通过增加钙瞬态来控制心跳强度，从而促进心肌细胞收缩。在这里，我们描述了一种在体外诱导拉伸介导的肥大后测量人心室心肌细胞线粒体Ca2+通量的方法。
Calcium Ca2+ regulation is a key component of numerous cellular functions. In cardiomyocytes, Ca2+ regulates excitation-contraction coupling and influences signaling cascades involved in cell metabolism and cell survival. Prolonged dysregulation of mitochondrial Ca2+ leads to dysfunctional cardiomyocytes, apoptosis and ultimately heart failure. VEGF promotes cardiomyocyte contractility by increasing calcium transients to control the strength of the heartbeat. Here, we describe a method to measure mitochondrial Ca2+ fluxes in human ventricular cardiomocytes after inducing stretch-mediated hypertrophy in vitro.