Residual extracellular polymeric substances (EPS) detected by fluorescence microscopy on dental implants after different decontamination
Residual extracellular polymeric substances (EPS) detected by fluorescence microscopy on dental implants after different decontamination
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DOI：
10.1016/j.matchemphys.2022.127242
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发表时间：
2022-12
影响因子：
4.6
通讯作者：
Ting Yang;Li Xie;Xingyue Hu;K. He;Zhuoli Zhu;Lin Fan;W. Tian
中科院分区：
文献类型：
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作者：
Ting Yang;Li Xie;Xingyue Hu;K. He;Zhuoli Zhu;Lin Fan;W. Tian
由于现有的检测技术无法区分细胞外聚合物（EPS）成分（多糖、蛋白质和核酸等）的类型，因此需要采用更有针对性的方法来检测净化后的牙种植体上残留的EPS成分。在此，我们的目的是评估荧光显微镜方法是否适用于检测净化后的牙种植体上残留的 EPS 成分。收集临床回收的6颗患有严重种植体周围炎的牙种植体，并制备金黄色葡萄球菌（S. aureus）培养的钛盘。去污（Er:YAG激光、空气磨料和柠檬酸）前后，用相应的荧光染料标记样品上的多糖、蛋白质和核酸进行识别，然后在共焦激光扫描显微镜（CLSM）下观察。此外，还使用了扫描电子显微镜（SEM）和X射线光电子能谱（XPS）检测技术。结果表明，去污前EPS成分的荧光信号较强，处理后荧光信号减弱，表明其强度可以大致反映含量。这项初步研究证明 EPS 染色荧光显微镜作为植入物去污结果评估的首选检测工具是可行的。然而，应进行进一步的研究，结合大样本量和量化数据。
Since the existing used detecting techniques were unable to distinguish the types of extracellular polymeric substances (EPS) components (polysaccharides, proteins, and nucleic acids et al.), it is needy to apply more targeted methods to detect the residual EPS components on decontaminated dental implants. Herein we aimed to assess whether fluorescence microscopy method was feasible for detecting residual EPS components on decontaminated dental implants. Six clinical-retrieved dental implants suffered severe peri-implantitis were collected, and also Staphylococcus aureus (S. aureus) cultured titanium discs were prepared. Before and after decontamination (Er:YAG laser, air-abrasive, and citric acid), corresponding fluorochrome stains were used to label polysaccharides, proteins, and nucleic acids on the samples for identification and then observed under confocal laser scanning microscopy (CLSM). Besides, scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS) detection techniques were also used. The results showed that fluorescent signals of EPS components are strong before decontamination and decreased after treatments, indicating that the intensity can roughly reflect the amounts. This preliminary study proved that EPS-stained fluorescence microscopy was feasible as a detection tool of choice for implant decontamination outcome evaluation. However, further study incorporating large sample size and quantified data should be carried out.