Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise
Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise
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DOI：
10.1096/fj.201700531r
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发表时间：
2017-12-01
期刊：
影响因子：
4.8
通讯作者：
Hawley, John A.
中科院分区：
文献类型：
--
作者：
Camera, Donny M.;Burniston, Jatin G.;Hawley, John A.
人们普遍认为，肌肉对阻力运动(REX)训练的适应是由收缩诱导的、蛋白质合成速率和食物蛋白质可获得性的增加所支撑的。通过使用动态蛋白质组图谱(DPP)，我们在逐个蛋白质的基础上研究了合成和分解对人类骨骼肌丰度变化的贡献。年龄匹配、超重的男性摄入高脂肪、低碳水化合物饮食9天，在此期间，他们要么进行3次REX，要么不进行锻炼。用质谱仪测定新合成的肌肉蛋白中前体物的富集率和氧化氢的掺入速率。DPP包括90个蛋白质，其中28个蛋白质对REX表现出显著的反应。最常见的反应模式是营业额增加，紧随其后的是丰度增加，但蛋白质合成没有明显增加。在这里，我们提供了新的证据，表明合成和分解对REX诱导的蛋白质丰度变化的贡献在蛋白质的基础上是不同的。我们还强调了运动后人体骨骼肌中个别肌肉蛋白质降解的重要性。
It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction-induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP), we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein-by-protein basis inhuman skeletal muscle. Age-matched, overweight males consumed 9 d of a high-fat, low-carbohydrate diet during which time they either undertook 3 sessions of REX or performed no exercise. Precursor enrichment and the rate of incorporation of deuterium oxide into newly synthesized muscle proteins were determined by mass spectrometry. Ninety proteins were included in the DPP, with 28 proteins exhibiting significant responses to REX. The most common pattern of response was an increase in turnover, followed by an increase in abundance with no detectable increase in protein synthesis. Here, we provide novel evidence that demonstrates that the contribution of synthesis and breakdown to changes in protein abundance induced by REX differ on a protein by-protein basis. We also highlight the importance of the degradation of individual muscle proteins after exercise in human skeletal muscle.