Organ pathology in the absence of bacteria?
Organ pathology in the absence of bacteria?
复制标题
没有细菌时的器官病理学？
DOI：
10.1093/infdis/jit606
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Niederweis,Michael
中科院分区：
文献类型：
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作者：
Jones,ChristopherM;Niederweis,Michael
致编辑-在Reddy等人[1]的文章中，描述了结核分枝杆菌的一种突变体，该突变体在感染豚鼠的几个器官中引起严重的病理学，而没有任何可检测到的细菌。Reddy等人构建了一个突变体，该突变体缺乏M.结核本研究的目的是检验铁磷修复的铁获得是否是M.结核病，因为它是许多其他细菌病原体[2，3]。类似于其他的M。在结核分枝杆菌素合成突变体中，ΔmbtE突变体不再产生铁载体，并且不能在低铁培养基中生长[4]。然后，作者用ΔmbtE突变体感染豚鼠，观察到与感染野生型M.结核病（图5 [1];见感染后4周的数据）。然而，当肺或脾匀浆在琼脂平板上孵育时，未检出细菌（图5;第9页[1]）。很难理解为什么mbtE突变细菌明显不能引起与大约100 000或10 000野生型M相同的严重器官病理学。肺和脾脏中的结核菌。对这一矛盾结果的简单解释是ΔmbtE突变体的细菌负荷与野生型M相似。结核病，因此引起类似的病理学，但ΔmbtE突变体不能在琼脂平板上生长，因为它不能使用没有铁载体的培养基中的铁盐[4]。事实上，我们经历过类似的问题
TO THE EDITOR—In the article by Reddy et al [1], a mutant of Mycobacterium tuberculosis is described that causes severe pathology in several organs of infected guinea pigs without any detectable bacteria. Here, we provide an alternative explanation for this observation without evoking homeopathy.Reddy et al constructed a mutant lacking one of the enzymes (MbtE) required for synthesis of siderophores in M. tuberculosis. The aim of this study was to examine whether siderophoremediated iron acquisition is a virulence trait of M. tuberculosis, as it is for many other bacterial pathogens [2, 3]. Similar to other M. tuberculosis mycobactin synthesis mutants, the ΔmbtE mutant did not produce siderophores anymore and did not grow in medium with low iron [4]. Then, the authors infected guinea pigs with the ΔmbtE mutant and observed the same pathology as in guinea pigs infected with wild-type M. tuberculosis (Figure 5 [1]; see data from 4 weeks after infection). However, no bacteria were detected when lung or spleen homogenates were incubated on agar plates (Figure 5; p. 9 [1]). It is difficult to understand how apparently no mbtE mutant bacteria can cause the same severe organ pathology as approximately 100 000 or 10 000 wild-type M. tuberculosis bacteria in the lungs and spleens, respectively, of the infected guinea pigs. A simple explanation for this contradictory result is that the bacterial load of the ΔmbtE mutant is similar to that of wild-type M. tuberculosis, consequently causing similar pathology, but the ΔmbtE mutant did not grow on agar plates because it cannot use the iron salts in the medium without siderophores [4]. In fact, we experienced a similar problem
糖蛋白和蛋白聚糖的生物化学
影响因子：
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作者：
W. Lennarz
通讯作者：
W. Lennarz