Analysis of anti-inflammatory dehydrodiisoeugenol and metabolites excreted in rat feces and urine using HPLC-UV
Analysis of anti-inflammatory dehydrodiisoeugenol and metabolites excreted in rat feces and urine using HPLC-UV
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HPLC-UV 分析大鼠粪便和尿液中的抗炎脱氢二异丁香酚及其代谢物
DOI：
10.1002/bmc.1717
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发表时间：
2012-06-01
影响因子：
1.8
通讯作者：
Yang, Xiu-Wei
中科院分区：
文献类型：
--
作者：
Li, Fei;Yang, Xiu-Wei
脱氢二异丁香酚（DDIE）是肉豆蔻果实中的一种木脂素。它在体外和体内代谢中可转化为多种代谢产物。在本研究中，研究了静脉（i. v.）和胃内（i.g.）给大鼠施用。采用HPLC法测定DDIE及其代谢产物（M-1和M-2）。DDIE及其代谢物在粪便中的排泄量高于尿液，表明DDIE及其代谢物主要在粪便中消除。在i. v.和i.g.之间观察到DDIE及其代谢物的排泄水平存在显著差异。局静脉内给药后排出的DDIE及其代谢产物量更大，表明DDIE在静脉内给药后可发挥更长时间的抗炎活性。局该方法的准确度、精密度、回收率和稳定性均满足大鼠尿液和粪便中DDIE及其代谢物的测定要求。本研究中的观察结果将有助于了解DDIE的吸收、分布、代谢和排泄途径，并有助于决策治疗期间DDIE给药的最佳模式，以最大限度地发挥其抗炎作用。版权所有（C）2011约翰威利父子有限公司
Dehydrodiisoeugenol (DDIE) is a lignan in the fruit of Myristica fragrans. It can be converted into several metabolites in in vitro and in vivo metabolism. In this study, the excretion of DDIE in urine and feces was investigated after intravenous (i.v.) and intragastric (i.g.) administration to rats. DDIE and its metabolites (M-1 and M-2) were measured using HPLC. The amount of DDIE and its metabolites excreted was higher in feces than in urine, suggesting that DDIE and its metabolites are eliminated primarily in the feces. Significant differences in the excretion levels of DDIE and its metabolites were seen between i.v. and i.g. administration. Greater amounts of DDIE and its metabolites were excreted following i.v. administration, suggesting that DDIE can exert a longer period of anti-inflammatory activity following i.g. administration. The accuracy, precision, recovery and stability of the analytical method in this study were satisfactory for the measurement of DDIE and its metabolites in rat urine and feces. Observations made in this study will contribute to understanding of the absorption, distribution, metabolism and excretion pathway of DDIE and will aid decision-making regarding the best mode of DDIE administration during treatment to maximize its anti-inflammatory effects. Copyright (C) 2011 John Wiley & Sons, Ltd.