Chitosan-Modified Stable Colloidal Gold Nanostars for the Photothermolysis of Cancer Cells
Chitosan-Modified Stable Colloidal Gold Nanostars for the Photothermolysis of Cancer Cells
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DOI：
10.1021/jp311271p
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发表时间：
2013-02-07
影响因子：
3.7
通讯作者：
Tsai, Din Ping
中科院分区：
文献类型：
--
作者：
Baginskiy, Ivan;Lai, Tsung-Ching;Tsai, Din Ping
报道了生物高分子壳聚糖修饰等离子体金纳米星（Au NS）的制备及其性质。将Au NS在生理pH 7.5下的胶体稳定性及其在体外癌细胞光热作用中的性能与金纳米棒（Au NRs）进行了比较。在pH = 7.5的介质中，壳聚糖修饰的Au纳米粒子的光学特性比壳聚糖修饰的纳米粒子的光学特性稳定，这是因为纳米粒子的聚集速度较慢。在pH = 7.5时，壳聚糖修饰的Au NR形成具有高度不均匀尺寸的聚集体。另一方面，Au NS形成小的链状簇，其中单个NS彼此连接，优选地通过分支与中心核的缔合。这是可能的面电荷密度的差异，在这些部分的NS是负责他们的首选协会。流式细胞术分析表明，由于NR的快速和不均匀聚集，与Au NS相比，壳聚糖封端的Au NR在整个细胞系中的分布相对不相等。对J5癌细胞的体外光热实验表明，分别用4 μ g/mL壳聚糖覆盖的Au NS和NR孵育的J5细胞需要23和33 mJ/cm（2）的能量通量才能完全死亡。当壳聚糖被用作表面封端剂时，Au NS在生理pH下表现出比NR更高的胶体稳定性，并且由于更均匀的细胞摄取，细胞光热所需的能量通量更低。
The preparation and properties of plasmonic gold nanostars (Au NSs) modified with a biopolymer chitosan are reported. The colloidal stability of Au NSs at the physiological pH of 7.5 and their performance in the photothermolysis of cancer cells in vitro were compared with those of gold nanorods (Au NRs). The optical characteristics of chitosan-modified Au NSs dispersed in a medium with pH = 7.5 had higher stability than those of chitosan-capped NRs because of the slower aggregation of NSs. At pH = 7.5, the chitosan-modified Au NRs formed aggregates with highly nonuniform sizes. On the other hand, Au NSs formed small chain-like clusters, in which individual NSs were connected to one another, preferably via association of branches with central cores. It is possible that the difference in areal charge density at these parts of NSs is responsible for their preferred association. Flow cytometry analysis showed the relatively nonequivalent distribution of the chitosan-capped Au NRs across the cell line compared with that of Au NSs because of the fast and nonuniform aggregation of NRs. An in-vitro photothermolysis experiment on J5 cancer cells showed that energy fluences of 23 and 33 mJ/cm(2) are necessary to cause complete death of J5 cells incubated with 4 mu g/mL chitosan-capped Au NSs and NRs, respectively. When chitosan was used as a surface-capping agent, the Au NSs exhibited higher colloidal stability at the physiological pH than the NRs and lower energy fluence necessary for cell photothermolysis because of more uniform cellular uptake.