Patterns of gene expression in developing anthers of Brassica napus
Patterns of gene expression in developing anthers of Brassica napus
复制标题
甘蓝型油菜花药发育中的基因表达模式
作者：
R. Scott;E. Dagless;R. Hodge;W. Paul;I. Soufleri;J. Draper
甘蓝型油菜（Brassica napus）的花药发育阶段与花蕾长度、花药长度之间的关系是通过一系列细胞学标记来确定的。已确定芽长与花药长直接相关，并且花药或芽长与花药内的雄配子发生阶段紧密相关。构建了两个花药cDNA文库，一个来自1.2-1.8 mm长花蕾的花药（孢子发生文库），一个来自1.8-4.0 mm长花蕾的花药（小孢子发育文库）。通过差异筛选共分离出19个独立的cDNA，其时间表达模式重叠，并且共同涵盖了从减数分裂前小孢子母细胞到三核花粉粒的花药发育阶段。这些克隆中的每一个的表达模式都是独特的，并且表明通过光学显微镜不能容易地区分的花药发育阶段可以通过某些RNA的存在或不存在来识别。从孢子发生文库中分离的三个cDNA经原位杂交证明是绒毡层特异的。相反，从小孢子发育文库中分离的5个克隆是小孢子特异的。这些克隆表现出不同的表达模式，以前描述的，他们的成绩单是不存在于成熟的花粉粒。因此，这些RNA可能是小孢子发育所必需的，而不是萌发花粉粒生长所必需的。
The relationship between bud length, anther length and stage of anther development has been investigated in Brassica napus using a series of cytological markers that define steps in the process of male gametogenesis. It was determined that bud length is directly related to anther length and that anther or bud length is tightly linked to the stage of male gametogenesis within the anther. This simple correlation has enabled the construction of cDNA libraries representing transcripts expressed in defined stages of anther development, and the detailed examination of the developmental pattern of expression of anther RNAs.Two anther cDNA libraries were constructed, one from anthers of 1.2–1.8 mm long buds (sporogenesis library) and one from anthers of 1.8–4.0 mm long buds (microspore development library). A total of 19 independent cDNAs have been isolated by differential screening whose temporal expression patterns overlap and which together cover the stages of anther development from pre-meiotic microsporocytes to tri-nucleate pollen grains. The pattern of expression of each of these clones is unique and indicates that stages of anther development which cannot be easily distinguished by light microscopy can be recognised by virtue of the absence or presence of certain RNAs. Three cDNAs isolated from the sporogenesis library have been shown by in situ hybridisation to be tapetum-specific. In contrast, five clones isolated from the microspore development library are microspore-specific. These clones exhibit a pattern of expression different to those previously described in that their transcripts are absent in mature pollen grains. Thus these RNAs are probably required in microspore development rather than for the growth of the germinating pollen grain.
影响因子：
11.6
作者：
BOWMAN, JL;SMYTH, DR;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM