Crystallographic analysis of the neurophysin-oxytocin complex. A preliminary report.
Crystallographic analysis of the neurophysin-oxytocin complex. A preliminary report.
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神经素-催产素复合物的晶体分析。
DOI：
10.1016/0022-2836(91)80201-5
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发表时间：
1991
影响因子：
5.6
通讯作者：
Wang,BC
中科院分区：
文献类型：
--
作者：
Rose,JP;Breslow,E;Huang,HB;Wang,BC
以硫酸铵为沉淀剂，得到牛垂体后叶素Ⅱ-催产素复合物的单晶。晶体的衍射分辨率至少为2.7 μ m，属于劳厄4/mm群，系统性缺失与空间群P41212或P43212一致。晶胞尺寸面积=B= 69·07 μ m，c = 113·26 μ m。晶体含有一个neurophysin-oxytocin二聚体每asymometric单位。以aVm = 2·903/Da计算，溶剂含量为58%。溶解的晶体的色谱分析表明每个neurophysin二聚体存在三个催产素分子。
Single crystals of a bovine neurophysin II-oxytocin complex have been obtained using (NH4)2SO4as the precipitating agent. The crystals diffract to at least 2·7Åresolution, belong to Laue group 4/mmmand exhibit systematic absences consistent with either space groupP41212 orP43212. The cell dimensions area=b= 69·07Å andc= 113·26Å. The crystals contain one neurophysin-oxytocin dimer per asmymmetric unit. Based on aVmof 2·9Å3/Da, the solvent content is calculated to be 58%. Chromatographic analysis of the dissolved crystals suggests the presence of three oxytocin molecules per neurophysin dimer.