"Natural" Human Leukocyte Antigen Antibodies Found in Nonalloimmunized Healthy Males
"Natural" Human Leukocyte Antigen Antibodies Found in Nonalloimmunized Healthy Males
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DOI：
10.1097/tp.0b013e318186d87b
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发表时间：
2008-10-27
期刊：
影响因子：
6.2
通讯作者：
Alberu, Josefina
中科院分区：
文献类型：
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作者：
Morales-Buenrostro, Luis E.;Terasaki, Paul I.;Alberu, Josefina
背景人类白细胞抗原（HLA）抗体通常不存在于未通过妊娠、输血或移植免疫的受试者中。但随着新的方法，我们现在看到，HLA抗体经常发现在非同种免疫的男性。用Luminex单抗原微球检测424例健康男性献血员血清。当荧光值大于1000作为临界值时，424名男性献血员中63%检测到人类白细胞抗原抗体。42%的人发现I类抗体，11%的人发现II类抗体，12%的人同时发现这两种抗体。在6个月内进行8次试验的5只雄性动物在每次试验中在相似强度水平下始终具有相同的特异性。这些抗体与一般人群中罕见的特异性反应：18.9%的人具有A*3002抗体;超过10%的人具有A*3101、B76、B*8201和Cw*1701抗体。大约一半的抗体供体有一个或两个特异性;另一半有三个或更多的特异性。在具有II类特异性的患者中，20.5%具有DPA 1 * 020 I/DPB 1 *0101抗体，10.8%具有DQA 1 *0503/DQB 1 *0301抗体。由于上述数据是通过检测424名墨西哥献血者的血清获得的，作为对照，对洛杉矶的29名男性进行了检测，结果显示在大致相似的频率下具有相似的特异性。正常男性被发现有罕见的HLA特异性的HLA抗体。这些HLA抗体很可能是针对微生物、摄入的蛋白质和过敏原中发现的交叉反应表位产生的，从而使它们成为天然抗体。
Background. Human leukocyte antigen (HLA) antibodies were normally not found in subjects who have not been immunized by pregnancies, transfusions, or transplants. But with new methodology, we now see that HLA antibodies are often found in nonalloimmunized males.Methods. The sera of 424 healthy male donors were tested with single antigen Luminex beads.Results. Human leukocyte antigen antibodies were detected in 63% of 424 male blood donors when a fluorescent value of more than 1000 was used as the cutoff. Antibodies to class I was found in 42%, class II in 11% and both in 12%. Five males who were tested eight times over a 6-month period consistently had the same specificity at similar strength levels at each testing. The antibodies reacted with specificities that are rare in the general population: 18.9% had antibodies to A*3002; more than 10% had antibodies to A*3101, B76, B*8201, and Cw*1701. About half of the donors with antibodies had one or two specificities; the other half had three or more specificities. Among those with class II specificities, 20.5% had antibodies to DPA1*020I/DPB1*0101, and 10.8% to DQA1*0503/DQB1*0301. Because the above data were obtained by testing sera of 424 Mexican donors, as a check, 29 males in Los Angeles were tested and shown to have similar specificities at roughly similar frequencies.Conclusions. Normal males were found to have HLA antibodies to infrequent HLA specificities. It is likely that these, HLA antibodies are produced to cross-reactive epitopes found in microorganisms, ingested proteins and allergens-making them natural antibodies.