Polymerization of recombinant tau core fragments in vitro and seeding studies in cultured cells.
Polymerization of recombinant tau core fragments in vitro and seeding studies in cultured cells.
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DOI：
10.3389/fnins.2023.1268360
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发表时间：
2023
影响因子：
4.3
通讯作者：
Giasson, Benoit I.
中科院分区：
文献类型：
--
作者：
Paterno, Giavanna;Bell, Brach M.;Riley-DiPaolo, Alexis;LaVoie, Matthew J.;Giasson, Benoit I.
关键词：
使用淀粉样蛋白荧光测定法和电子显微镜研究了定义阿尔茨海默病、皮克病和皮质基底节变性的特定tau蛋白核心的相对聚合。此外，在基于细胞的测定中研究了由这些各自的tau蛋白片段组成的聚合物的相对朊病毒样活性。它表明，特定的tau核心原纤维的播种活动的存在下的致病性tau错义突变和微管结合结构域组成的tau的影响。还通过在阿尔茨海默病tau核心原纤维的存在下改变磷酸模拟残基和磷酸无效残基的延伸来研究tau磷酸化对接种倾向的独特影响。这些结果对突变和异构体特异性驱动的发病机制具有重要的机制意义。
The relative polymerization of specific tau protein cores that define Alzheimer’s disease, Pick’s disease and corticobasal degeneration were investigated using amyloid fluorometry and electron microscopy. In addition, the relative prion-like activities of polymers comprised of these respective tau protein segments were investigated in a cell-based assay. It is demonstrated that the seeding activities of specific tau core fibrils are affected by the presence of pathogenic tau missense mutations and the microtubule binding domain composition of tau. The unique impact of tau phosphorylation on seeding propensity was also investigated by altering stretches of phospho-mimetic and phospho-null residues in the presence of Alzheimer’s disease tau core fibrils. These results have important mechanistic implications for mutation and isoform-specific driven pathogenesis.
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影响因子：
2.9
作者：
Dinkel, Paul D.;Siddiqua, Ayisha;Margittai, Martin
通讯作者：
Margittai, Martin
影响因子：
16.2
作者：
GOEDERT, M;SPILLANTINI, MG;CROWTHER, RA
通讯作者：
CROWTHER, RA
影响因子：
11.4
作者：
GOEDERT, M;SPILLANTINI, MG;CROWTHER, RA
通讯作者：
CROWTHER, RA
影响因子：
16.2
作者：
GOEDERT, M;SPILLANTINI, MG;CROWTHER, RA
通讯作者：
CROWTHER, RA
影响因子：
11.4
作者：
GOEDERT, M;JAKES, R
通讯作者：
JAKES, R