Seroprevalence of Angiostrongylus cantonensis in Wild Rodents from the Canary Islands
Seroprevalence of Angiostrongylus cantonensis in Wild Rodents from the Canary Islands
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DOI：
10.1371/journal.pone.0027747
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发表时间：
2011-11-14
期刊：
影响因子：
3.7
通讯作者：
Valladares, Basilio
中科院分区：
文献类型：
--
作者：
Martin-Alonso, Aaron;Foronda, Pilar;Valladares, Basilio
背景：广州管圆线虫是鼠科鼠肺线虫，是人类脑管圆线虫病的病原体。生活史研究表明，A.广州线虫分别以大鼠和软体动物为最终宿主和中间宿主。本研究旨在增加对该虫的发生和分布的认识。cantonensis在其最终的主机在加那利群岛，在不同地区和年龄组的寄生虫学和血清学分析方法/主要调查结果：在2009年和2010年之间，54黑鼠（Rattus rattus）从特内里费岛被捕获的六个人类居住区和血清样本。经形态学和分子生物学鉴定，肺线虫为A.广东话。从A.用电泳和电洗脱法对广州管圆线虫蠕虫进行了鉴定。54只鼠中有30只鼠出现抗A.用ELISA法检测广州管蚜31-kDa抗原。因此，总体血清阳性率为55.6%（95% CI：42.4-68）。6个地区均发现血清阳性鼠。这种31-kDa抗原不被感染其他蠕虫物种（但不是A. cantonensis）。抗A.广州线虫感染率与成虫感染率呈显著正相关（R-2 = 0.954，p
Background: Angiostrongylus cantonensis is a lungworm of rats (Muridae) that is the causative agent of human cerebral angiostrongyliasis. The life cycle of A. cantonensis involves rats and mollusks as the definitive and intermediate hosts, respectively. This study was designed to increase the knowledge about the occurrence and distribution of A. cantonensis in its definitive host in the Canary Islands, using parasitological and serological analysis in different areas and age groups.Methodology/Principal Findings: Between 2009 and 2010, 54 black rats (Rattus rattus) from Tenerife were captured from six human-inhabited areas and sera samples were obtained. The lung nematodes were identified by morphological and molecular tools as A. cantonensis. The 31-kDa glycoprotein antigen was purified from A. cantonensis adult worms by electrophoresis and electroelution. Of the 54 tested rodents, 30 showed IgG antibodies against A. cantonensis 31-kDa antigen by ELISA. Therefore, the overall seroprevalence was 55.6% (95% CI: 42.4-68). Seroprevalent rodents were found in all the 6 areas. This 31-kDa antigen was not recognized by some sera of rats infected by other helminth species (but not A. cantonensis). Seroprevalence of IgG antibodies against A. cantonensis and prevalence based on the presence of adult worms showed significant correlation (R-2 = 0.954, p