Heritability of resistance-related gene expression traits and their correlation with body size of clam Meretrix petechialis
Heritability of resistance-related gene expression traits and their correlation with body size of clam Meretrix petechialis
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文蛤抗性相关基因表达性状遗传力及其与体型的相关性
DOI：
10.1007/s00343-019-8326-3
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发表时间：
2020-03-01
影响因子：
1.6
通讯作者：
Wang, Hongxia
中科院分区：
文献类型：
--
作者：
Jiang, Fengjuan;Yue, Xin;Wang, Hongxia
基因表达变异可以被认为是一种表型，它在驯化和适应中都起着重要作用。然而，与传统的商业性状相比，基因表达的遗传变异在水产养殖中受到的关注较少。为研究蛤蜊斑点Meretrix petechialis基因转录的遗传变异和遗传力，选择5个抗性弧菌相关基因在消化腺中进行基因表达分析，采用动物线性模型对实时荧光定量PCR （qRT-PCR）数据进行分析。5个基因中，BIRC7基因表现出显著的加性遗传变异，12月龄和15月龄蛤的遗传率分别为0.84±0.32和0.91±0.34。其他4个基因（Bax、NFIL3、Big-Def和CTL9）的遗传率为中低表达，但未显著表达。此外，未发现BIRC7转录性状与体型之间存在显著的表型和遗传相关性。本研究强调了某些基因表达变异具有遗传性，为间接选择具有高弧菌抗性的斑点分枝杆菌提供了参考。
Gene expression variation can be considered as a phenotype, and it plays an important role in both acclimation and adaption. However, genetic variation of gene expression received much less attention than traditional commercial traits in aquaculture. To estimate the genetic variation and heritability of gene transcription in clam Meretrix petechialis, five Vibrio resistance-related genes were selected for gene expression analysis in the digestive gland, and an animal linear model was used to analyze data from quantitative real-time PCR (qRT-PCR). Among the five genes, BIRC7 showed significant additive genetic variations, the heritability of this gene of 12-month- and 15-month-old clams were 0.84±0.32 and 0.91±0.34, respectively. The heritability of other four genes (Bax, NFIL3, Big-Def, and CTL9) expression were low-to-moderate but not significantly expressed. Additionally, no significant phenotypic and genetic correlations between the BIRC7 transcription trait and body size were detected. This study highlights that certain gene expression variation is heritable and provides a reference for indirect selection of M. petechialis with high Vibrio resistance.