Increased lentivirus titer using ultra expression vectors
Increased lentivirus titer using ultra expression vectors
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使用超表达载体提高慢病毒滴度
DOI：
10.1016/j.ab.2023.115119
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发表时间：
2023
影响因子：
2.9
通讯作者：
Fukuda Tomokazu
中科院分区：
文献类型：
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作者：
Fujibayashi So;Kiyono Tohru;Endo Yuka;Tani Tetsuya;Tate Haruka;Bai Lanlan;Sugano Eriko;Tomita Hiroshi;Fukuda Tomokazu
慢病毒是一种高效的基因转移系统，广泛应用于基础科学领域。我们的目的是通过将超表达载体应用于慢病毒包装来提高病毒滴度。超表达载体的应用使标准制剂的生物效价提高了4倍。我们还评估了超表达载体对相对较长的插入片段的效力，例如在多个克隆位点中含有5个基因的CSII-CMV-CNROE。包装的超表达载体的生物效价比原方法高3.5倍。我们改进的包装系统可以应用于慢病毒，以产生更高的滴度。
Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers.