Studies of the effect of Clostridium butyricum on Helicobacter pylori in several test models including gnotobiotic mice
Studies of the effect of Clostridium butyricum on Helicobacter pylori in several test models including gnotobiotic mice
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DOI：
10.1099/0022-1317-49-7-635
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发表时间：
2000-07-01
影响因子：
3
通讯作者：
Kamiya, S
中科院分区：
文献类型：
--
作者：
Takahashi, M;Taguchi, H;Kamiya, S
在体内外研究了酪酸梭菌与幽门螺杆菌的相互作用。米氏丁酸菌588的培养上清液在调节pH值为7.4时仍能抑制幽门螺杆菌的生长。丁酸对幽门螺杆菌的杀菌作用强于乳酸、醋酸和盐酸。流式细胞仪分析显示，胃上皮细胞(MKN45)与H、Pylori和C，Buyricum预先孵育可抑制H、Pylori与MKN45细胞的黏附。无菌小鼠胃粘膜持续感染幽门螺杆菌5周。致病小鼠感染丁酸菌后可治愈幽门螺杆菌持续感染，益生菌C-Miyairi 588可能对幽门螺杆菌感染有一定的治疗作用。
The interaction between Clostridium butyricum and Helicobacter pylori was examined in vitro and in vivo. The culture supernate of C, butyricum MIYAIRI 588 inhibited the growth of H. pylori even when its pH was adjusted to 7.4. The bactericidal effect of butyric acid on H. pylori was stronger than that of lactic, acetic or hydrochloric acids. Flow cytometric analysis showed that pre-incubation of gastric epithelial (MKN45) cells with H, pylori and C, butyricum inhibited the adhesion of H, pylori to the cells. Persistent infection with H, pylori in the gastric mucosa of germ-free mice was observed for 5 weeks. Cure of persistent infection with H, pylori in the gnotobiotic mice was demonstrated following infection with C, butyricum, The probiotic agent, C, butyricum MIYAIRI 588 may have some beneficial effects on H, pylori infection.