Promoter opening (melting) and transcription initiation by RNA polymerase I requires neither nucleotide beta,gamma hydrolysis nor protein phosphorylation.
Promoter opening (melting) and transcription initiation by RNA polymerase I requires neither nucleotide beta,gamma hydrolysis nor protein phosphorylation.
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RNA 聚合酶 I 启动子打开（解链）和转录启动既不需要核苷酸 β、γ 水解，也不需要蛋白质磷酸化。
DOI：
10.1093/nar/21.14.3233
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发表时间：
1993
影响因子：
14.9
通讯作者：
Paule,MR
中科院分区：
文献类型：
--
作者：
Lofquist,AK;Li,H;Imboden,MA;Paule,MR
With some bacterial RNA polymerases and In eukaryotic RNA polymerase II, DNA melting during Initiation requires the coupling of energy derived fromβ,γhydrolysis of ATP. A detailed analysis of this possible requirement for eukaryotic RNA polymerase I reveals no such requirement. However, in some cases,β,γnon-hydrolyzable derivatives (β,γImido or methylene) of nucleotide substrates have been found to significantly Inhibit transcription initiation because of their inefficient use as the first nucleotlde of the transcript. in addition, the results presented here show that protein kinase activity Is not requIred as an integral part of transcription initiation by RNA polymerase I. Prior phosphorylatlon of proteins participating in the process is not ruled out.
