xlgv7: a maternal gene product localized in nuclei of the central nervous system in Xenopus laevis.
xlgv7: a maternal gene product localized in nuclei of the central nervous system in Xenopus laevis.
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xlgv7：一种位于非洲爪蟾中枢神经系统细胞核中的母体基因产物。
DOI：
10.1101/gad.3.4.572
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发表时间：
1989
影响因子：
10.5
通讯作者：
Etkin,L
中科院分区：
文献类型：
--
作者：
Miller,M;Kloc,M;Reddy,B;Eastman,E;Dreyer,C;Etkin,L
爪蟾卵母细胞核（GV）是早期发育过程中使用的大量蛋白质的仓库。我们克隆并鉴定了编码母体基因产物的 cDNA，该产物位于 GV 中，然后在蝌蚪和成年青蛙的中枢神经系统 (CNS) 的细胞核中高度富集。该 cDNA (xlgv7) 为 2.1 kb，在 Northern 印迹上与 2.4 kb RNA 物种杂交。基因组 DNA 的 Southern 印迹表明该基因是多基因家族的成员。 cDNA 序列揭示了 1773 个核苷酸的长开放阅读框 (ORF)，在最末端羧基末端具有推定的核靶向信号 (Glu Arg Arg Lys Lys Lys Thr)，内部富含组氨酸 (His) 区域，His 对之间具有重复的保守氨基酸序列。该区域的意义尚不清楚，但该蛋白是DNA结合蛋白，该区域有可能参与该功能。 xlgv7 蛋白还具有与细菌 RecA 和 RecB 以及酵母 RAD 蛋白相似的假定核苷酸结合共有序列。蛋白质 xlgv7 以几种同种型的形式存在，在发育过程中表现出发育和细胞特异性的变化。 xlgv7 mRNA 丰度的 Northern 印迹分析显示在第 15-16 阶段神经诱导后出现积累。 mRNA 在蝌蚪肠道中短暂表达。在成人中，mRNA 在大脑中高度富集，而在其他组织中不存在或丰度非常低。该蛋白质的免疫组织化学分析表明该蛋白质位于脑细胞的细胞核中。我们得出的结论是，xlgv7 基因产物是一种母体蛋白，可能具有多种重要功能，其中之一可能是中枢神经系统的发育和维护。
The Xenopus oocyte nucleus (GV) is a storehouse for a large number of proteins that are used during early development. We have cloned and characterized a cDNA coding for a maternal gene product that is localized in the GV and then becomes highly enriched in the nuclei of the central nervous system (CNS) of tadpoles and adult frogs. This cDNA (xlgv7) is 2.1 kb and hybridizes to a 2.4-kb RNA species on Northern blots. Southern blots of genomic DNA suggest that this gene is a member of a multigene family. The cDNA sequence reveals a long open reading frame (ORF) of 1773 nucleotides, with a putative nuclear targeting signal (Glu Arg Arg Lys Lys Lys Thr) at the extreme carboxyl terminus and an internal histidine (His)-rich region with a repeated conserved amino acid sequence between His pairs. The significance of this region is unclear, but the protein is a DNA-binding protein, and it is possible that this region is involved in this function. The xlgv7 protein also possesses a putative nucleotide-binding consensus sequence that is similar to the bacterial RecA and RecB and yeast RAD proteins. Protein xlgv7 exists as several isotypes that exhibit developmental and cell-specific changes during development. Northern blot analysis of the abundance of the xlgv7 mRNA shows an accumulation following neural induction at stages 15-16. There is a transient expression of the mRNA in the gut of tadpoles. In the adult, the mRNA is highly enriched in the brain and is absent or in very low abundance in other tissues. Immunohistochemical analysis of the protein shows that the protein is localized in the nuclei of the brain cells. We conclude that the xlgv7 gene product is a maternal protein that may serve several important functions, one of which may be in the development and maintanance of the CNS.
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将海胆组蛋白基因注入非洲爪蟾卵母细胞，合成真正的海胆转录和翻译产物。
影响因子：
2.7
作者：
L. Etkin;R. Maxson
通讯作者：
R. Maxson
DOI：
10.1073/pnas.74.11.4835
发表时间：
1977-01-01
影响因子：
11.1
作者：
MCMASTER, GK;CARMICHAEL, GG
通讯作者：
CARMICHAEL, GG
“一种将 DNA 限制性内切酶片段放射性标记至高比活性的技术”。
影响因子：
2.9
作者：
Feinberg,AP;Vogelstein,B
通讯作者：
Vogelstein,B
DOI：
10.1073/pnas.82.4.1074
发表时间：
1985-01-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
DOI：
--
发表时间：
1985
期刊：
Cold Spring Harbor Symposia on Quantitative Biology
影响因子：
--
作者：
J. Gurdon
通讯作者：
J. Gurdon