Fibronectin production by cultured human trabecular meshwork cells.
Fibronectin production by cultured human trabecular meshwork cells.
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培养的人小梁网细胞产生纤连蛋白。
DOI：
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发表时间：
1982
影响因子：
4.4
通讯作者：
P. Cleveland
中科院分区：
文献类型：
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作者：
D. Worthen;P. Cleveland
23只眼库眼的人小梁网死后外植体的生长率为32%（71/160），其中8%（12）显示大量细胞扩散。来自11至40岁供体的眼睛实现了100%融合。其中一种培养物生长旺盛，我们能够通过八次培养来研究它。该培养物具有Polansky等人描述的培养人小梁网细胞的形态学特征。组织培养上清液中纤连蛋白的灵敏放射免疫分析显示，纤连蛋白分泌增加超过96小时的时间内，与特征性的下移反应发生在24和48小时后媒体的变化。四个培养物中的三个显示存在层粘连蛋白，一种基底膜蛋白，确定细胞不是成纤维细胞。
Postmortem explants of human trabecular meshwork from 23 eyebank eyes had a growth rate of 32% (71/160) cultures, with 8% (12) showing abundant cellular spread. One hundred percent confluency was achieved with eyes from donors of 11 to 40 years of age. One of the cultures grew profusely and we were able to study it through eight passages in culture. This culture had the morphologic characteristics described by Polansky et al. for cultured human trabecular meshwork cells. A sensitive radioimmunoassay for fibronectin in tissue culture supernatant showed an increase in fibronectin secretion over a 96 hr period, with a characteristic shift-down response occurring between 24 and 48 hr postmedia change. Three of four cultures showed the presence of laminin, a basement membrane protein, establishing that the cells are not fibroblasts.