An adipocyte-derived plasma protein, adiponectin, adheres to injured vascular walls
An adipocyte-derived plasma protein, adiponectin, adheres to injured vascular walls
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DOI：
10.1055/s-2007-978586
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发表时间：
2000-02-01
影响因子：
2.2
通讯作者：
Matsuzawa, Y
中科院分区：
文献类型：
--
作者：
Okamoto, Y;Arita, Y;Matsuzawa, Y
脂肪组织分泌多种蛋白质进入血流我们以前报道了一种新的cDNA，apM1（adipose most abundant gene transcript 1），它在脂肪组织中特异性和大量表达[1]。一级结构分析表明，apM1基因产物与VIII型胶原、X型胶原和补体因子Clq具有高度同源性，我们将其命名为脂联素。在本研究中，我们分析了脂联素在体外和体内的特性。将该cDNA转染COS细胞后，证实脂联素蛋白能分泌到培养液中。在人血浆中大量检测到抗脂联素的交叉反应性。在固相结合试验中，脂联素特异性结合存在于血管内膜中的1型、III型和V型胶原。免疫组化分析显示，脂联素检测到导管损伤的血管壁，但在完整的血管壁。这些数据表明，脂联素是由脂肪组织产生的血浆蛋白，当内皮屏障受损时在血管壁中积累。
Adipose tissue secretes a variety of proteins into the bloodstream We have previously reported a novel cDNA, apM1 (adipose most abundant gene transcript 1), which is specifically and abundantly expressed in adipose tissue [1]. Primary structure analysis predicted that the apM1 gene product possesses significant homology to collagens VIII, X and complement factor Clq, and we named it adiponectin. In the current study, we analyzed characteristics of adiponectin in vitro and in vivo. Adiponectin protein was proved to be secreted into the medium when the cDNA was transfected to COS cells. Anti-adiponectin crossreactivities were abundantly detected in the human plasma, In solid-phase binding assays, adiponectin specifically bound to collagen types 1, III and V, which are present in vascular intima. Immunohistochemical analysis revealed that adiponectin was detected in the walls of the catheter-injured vessels but not in the intact vascular walls. These data suggest that adiponectin is a plasma protein produced by adipose tissue and accumulates in vascular walls when the endothelial barrier is injured.