Raised tissue copper levels in oral submucous fibrosis.
Raised tissue copper levels in oral submucous fibrosis.
复制标题
口腔粘膜下纤维化中组织铜水平升高。
DOI：
10.1034/j.1600-0714.2000.290601.x
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
N. Johnson
中科院分区：
文献类型：
--
作者：
C. Trivedy;K. Warnakulasuriya;T. Peters;R. Senkus;V. Hazarey;N. Johnson
口腔粘膜下纤维化(OSF)是一种公认的口腔潜在恶性疾病，与咀嚼槟榔有关。与其他常吃的坚果相比，槟榔的铜含量很高，咀嚼槟榔5-30分钟会显著增加全口液中的可溶性铜。我们的目的是确定OSF患者的组织和血清铜浓度是否因咀嚼槟榔而升高。来自印度那格浦尔的OSF患者的一组口腔粘膜活检被用质谱仪(MAS)测量组织中铜的浓度。MAS法显示，11例OSF患者组织铜平均含量为5.5+/-2.9微克/克，而非槟榔咀嚼对照组(7例)组织铜平均含量为4+/-1.9微克/克(P=0.2)。用能量色散X射线微区分析(EDX)确定了金属元素的存在和分布。EDX在口腔黏膜上皮(21/23)和结缔组织(17/23)中显示出明显的铜(Kalpha8.04kev；Kbeta，8.91kev)峰，与非槟榔咀嚼受试者(n=7)的对照口腔活检所获得的光谱相比较。这些发现被少量样品的二次离子质谱仪(SIMS)分析所证实。14例OSF患者的血铜(17.23+/-1.80pmol/L)、铜蓝蛋白(0.32+/-0.04g/L)和尿铜(0.52+/-0.26微摩尔/L)均在实验室参考范围内。OSF组织中铜的发现支持铜在OSF中作为启动因子的假说，铜通过上调赖氨酸氧化酶活性而在刺激纤维化形成中发挥作用。
Oral submucous fibrosis (OSF) is a well-recognised-potentially malignant condition of the oral cavity associated with areca nut chewing. Areca nut has been shown to have a high copper content compared to other commonly eaten nuts, and chewing areca nut for 5-30 min significantly increases soluble copper in whole mouth fluids. Our aims were to determine if tissue and serum concentrations of copper were raised in patients with OSF as a result of chewing areca nut. A panel of buccal mucosal biopsies from patients with OSF from Nagpur, India, was used to measure the tissue concentrations of copper by mass absorption spectrometry (MAS). By MAS, the mean tissue copper level was 5.5+/-2.9 microg/g in the OSF specimens (n=11) compared with 4+/-1.9 microg/g in the non-areca chewing controls (n=7) (P=0.2). Energy dispersive x-ray microanalysis (EDX) was used to identify the presence and distribution of the metal element. EDX showed distinct peaks corresponding to copper (Kalpha 8.04 keV; Kbeta, 8.91 keV) in the epithelium (21/23) and in the connective tissue (17/23) of the OSF specimens compared to spectra obtained from control oral biopsies from non-areca chewing subjects (n=7). These findings were confirmed by secondary ion mass spectrometry (SIMS) analysis in a small number of samples. Serum copper (17.23+/-1.80 pmol/l), caeruloplasmin (0.32+/-0.04 g/l) levels and urinary copper (0.52+/-0.26 micromol/l) in OSF patients (n=14) were within the laboratory reference ranges. The finding of copper in OSF tissue supports the hypothesis of copper as an initiating factor in OSF, playing a role in stimulating fibrogenesis by the upregulation of lysyl oxidase activity.