Simvastatin affect the expression of detoxification-related genes and enzymes in Daphnia magna and alter its life history parameters
Simvastatin affect the expression of detoxification-related genes and enzymes in Daphnia magna and alter its life history parameters
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辛伐他汀影响大型溞解毒相关基因和酶的表达并改变其生活史参数
DOI：
10.1016/j.ecoenv.2019.109389
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发表时间：
2019-10-30
影响因子：
6.8
通讯作者：
Nie, Xiangping
中科院分区：
文献类型：
--
作者：
Liu, Yang;Ding, Rui;Nie, Xiangping
辛伐他汀(辛伐他汀，SV)作为一种降胆固醇药物，已在多种水环境中被检测到。然而，关于SV对淡水无脊椎动物的影响的信息有限。在本研究中，我们研究了SV对水蚤的毒性效应。在21d的慢性毒性试验中，我们还测定了7个与解毒和生殖相关的基因(即HR96、P-gp、CYP360A8、GST、CYP314、ECR和VTG)和几种酶(APND、ERND、GST和CAT)的表达。结果表明，高浓度(如50 mU g L-1)的SV短时间暴露(如24 h)可显著诱导巨藻体内HR96和P-gp的表达(最高可达2.5倍)和酶的表达(如ERND和GST活性增加4.0倍)。长期的慢性暴露(21天)可能会引起生活史参数的变化，如个体总产卵量和内在生长率的下降等。SV可能是一个潜在的内分泌干扰因子，繁殖参数是比存活和生长更敏感的终点。
Simvastatin (SV), as an hypocholesterolaemic drug, has been detected in various aquatic environment. However, limited information is available on the effects of SV on freshwater invertebrates. In the present study, we investigated the toxic effects of SV on Daphnia. magna (D. magna) through measuring the physiological changes (e.g., survival, growth rate, and reproduction) in a 21-d chronic toxicity test We also determined the expression of seven detoxification and reproduction-related genes (i.e. HR96, P-gp, CYP360A8, GST, CYP314, EcR and Vtg) and several enzymes (i.e. APND, ERND, GST and CAT) in a acute test (24 h). Results showed that high concentration (e.g. 50 mu g L-1) of SV for short time exposure (e.g. 24 h) significantly induced the expression of HR96 and P-gp (e.g. up to 2.5 folds)and enzymes (e.g. increasing 4.0 folds for ERND and GST activity) in D. magna.. The long-term chronic exposure (21 days) may cause the changes of life history parameters such as decreasing total egg production number per individual and intrinsic growth rates etc. SV may act as a potential endocrine disruptor to D. magna and the reproduction parameters were more sensitive endpoints than the survival and growth for evaluating SV exposure.